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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 3

sp. nov., a flavobacterium isolated from surface seawater Free

Abstract

A taxonomic study was carried out on strain SH27, which was isolated from seawater collected around Xiaoshi Island, PR China. Cells of strain SH27 were Gram-stain-negative, non-motile, rod-shaped, orange-pigmented and grew at 15–37 °C (optimum, 28 °C), at pH 6.0–8.0 (pH 7.0) and in 1.0–7.0 % (w/v) NaCl (2.0–3.0 %). The isolate was positive for catalase, but negative for nitrate reduction, oxidase, indole production and urease. Carotenoid pigment was produced. Phylogenetic analysis based on the 16S rRNA gene placed strain SH27 in the genus with the closest relative being KCTC 12391, exhibiting 96.7 % 16S rRNA gene pairwise similarity. The results of genomic comparisons, including average nucleotide identity and digital DNA–DNA hybridization, showed 72.9 and 19.2 % identity to KCTC 12391, respectively. The major cellular fatty acids were iso-C, iso-C G and iso-C 3-OH. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. Menaquinone-6 was the only respiratory quinone. The G+C content of the genomic DNA was 32.9 mol%. On the basis of the phenotypic and phylogenetic data, strain SH27 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain SH27 (MCCC 1H00358=CCTCC AB 2018323=KCTC 62962).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): digital DNA–DNA hybridization , Dokdonia sinensis and draft genome sequencing
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41876166)
    • Principle Award Recipient: Liu-Yan Zhou
© 2020 The Authors
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2020-01-02
2024-04-24
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Dokdonia sinensis sp. nov., a flavobacterium isolated from surface seawater
Int J Syst Evol Microbiol 70, 1617 (2020); https://doi.org/10.1099/ijsem.0.003949
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