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Volume 70, Issue 4

sp. nov., isolated from faeces of Tibetan antelope () on the Qinghai–Tibet Plateau Free

Abstract

Two Gram-stain-positive, catalase-positive and oxidase-negative, aerobic, non-motile, cellobiose-utilizing, short-rod-shaped strains (Z28 and Z29) were isolated from faeces of Tibetan antelope () collected on the Qinghai–Tibet Plateau. Strain Z28 shared 98.1, 98.0, 97.8 and 97.4 % 16S rRNA gene similarity, 24.1, 22.8, 23.2 and 26.3 % digital DNA–DNA hybridization relatedness and 80.8, 80.0, 80.7 and 80.9 % average nucleotide identity values with DSM 24482, DSM 20109, DSM 14785 and JCM 14899, respectively. Results from further phylogenetic analyses based on the 16S rRNA gene and 148 core genes indicated that strains Z28 and Z29 were closest to DSM 24482 and DSM 20109, but clearly separated from the currently recognized species of the genus . The genomic DNA G+C content of strain Z28 was 75.3 mol%. The major cellular fatty acids were -C, -C A, C and -C. Ribose and mannose were detected as the whole-cell sugars. The major respiratory quinone was MK-9(H) and ornithine was the diamino acid of the cell wall. The polar lipids present in strain Z28 were phosphatidylethanolamine, five phospholipids, two aminophospholipids, aminolipid and three unidentified lipids. Comparison of phenotypic and phylogenetic features between the two strains and the related organisms revealed that Z28 and Z29 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Z28 (=CGMCC 1.16477=DSM 106200).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Cellulomonas shaoxiangyii , Qinghai–Tibet Plateau and Tibetan antelopes (Pantholops hodgsonii)
Funding
This study was supported by the:
  • Chinese Academy of Medical Sciences and Sanming Project of Medicine in Shenzhen (Award SZSM201811071)
    • Principle Award Recipient: Jianguo Xu
  • Research Units of Discovery of Unknown Bacteria and Function (Award 2018RU010)
    • Principle Award Recipient: Jianguo Xu
  • National Science and Technology Major Project of China (Award 2018ZX10305409-003)
    • Principle Award Recipient: Zhi-hong Ren
  • National Science and Technology Major Project of China (Award 2018ZX10712001-007)
    • Principle Award Recipient: Jing Yang
© 2020 The Authors
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2020-02-10
2024-04-19
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Cellulomonas shaoxiangyii sp. nov., isolated from faeces of Tibetan antelope (Pantholops hodgsonii) on the Qinghai–Tibet Plateau
Int J Syst Evol Microbiol 70, 2204 (2020); https://doi.org/10.1099/ijsem.0.003939
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