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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 2

sp. nov., isolated from sediment of sea cucumber culture pond Free

Abstract

A Gram-stain-positive, rod-shaped (0.3–0.4×1.2–2.0 µm), strictly aerobic and beige-pigmented bacterium, designated B3227, was isolated from the sediment of a sea cucumber culture pond in Rongcheng, China (122.2° E 36.9° N). Its biochemical characteristics analysis revealed that the cells of this bacterium were catalase-positive and oxidase-negative. Cell growth occurred at 15–45 °C (optimum, 37 °C), pH 6.5–9.0 (pH 7.5–8.0) and in the presence of 0.0–22.0 % (w/v) NaCl (6.0–9.0 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain B3227 exhibited similarities of 95.7, 95.5, 95.5 and 95.3 % to the type strains of , , and , respectively, and the results of physiological analyses revealed that strain B3227 was most similar to the genus . The cells were endospore-forming and comprised an A1-γ--diaminopimelic acid-type peptidoglycan. The respiratory quinone of strain B3227 was MK-7, and the dominant fatty acids were anteiso-C and anteiso-C. The major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The genomic DNA G+C content was 38.7 mol%. The average nucleotide identity values between strain B3227 and JCM 14192 (ANIb 69.5%, ANIm 84.2 %) and JCM 12288 (ANIb 70.1 %, ANIm 84.1 %) were below the cut-off level (95–96  %) for species delineation. The results of analysis revealed that strain B3227 could biosynthesize shikimate acid, a base compound for the formulation of the swine flu drug. Based on its morphological and physiological properties, as well as phylogenetic distinctiveness, strain B3227 should be placed into the genus as a representative of a new species, for which the name sp. nov. is proposed. The type strain is B3227 (=KCTC 33093=MCCC 1H00193).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): 16S rRNA gene , Halalkalibacillus and phylogenetic analysis
Funding
This study was supported by the:
  • Science and Technology Basic Resources Investigation Program of China (Award 2017FY100300)
    • Principle Award Recipient: Zong-Jun Du
  • National Natural Science Foundation of China (CN) (Award 31770002)
    • Principle Award Recipient: Zong-Jun Du
© 2020 The Authors

Erratum

An erratum has been published for this content:
Corrigendum: sp. nov., isolated from sediment of sea cucumber culture pond
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2019-12-20
2024-04-19
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Halalkalibacillus sediminis sp. nov., isolated from sediment of sea cucumber culture pond
Int J Syst Evol Microbiol 70, 1398 (2020); https://doi.org/10.1099/ijsem.0.003927
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