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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 2

sp. nov., isolated from a freshwater river Free

Abstract

An isolate, designated TTM-7, recovered from a freshwater river in Taiwan, was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set (92 protein clusters) indicated that strain TTM-7 is affiliated with species in the genus . Strain TTM-7 was most closely related to NJ-8 with a 98.2 % 16S rRNA gene sequence similarity, followed by H32-4 (97.4 %) and SS2-56 (96.3 %). Cells were Gram-stain-negative, aerobic, motile by gliding, rod-shaped and formed orange colonies. Optimal growth occurred at 25 °C, pH 7 and 0 % NaCl. The major fatty acids of strain TTM-7 were iso-C, summed feature 3 (comprising Cωc/Cω and/or iso-C 2-OH) and iso-C G. The predominant hydroxy fatty acid was iso-C 3-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, two unidentified aminoglycolipids, five unidentified aminophospholipids and one unidentified lipid. The major isoprenoid quinone was MK-7. Genomic DNA G+C content of strain TTM-7 was 40.6 mol%. Strain TTM-7 showed 83.6 % average nucleotide identity and 16.0 % digital DNA–DNA hybridization identity with NJ-8. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain TTM-7 should be classified in a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TTM-7 (=BCRC 81159=LMG 30926=KCTC 62870).

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  • Accepted:
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  • Published Online:
Keyword(s): Chitinophagaceae , Chitinophagales , Chitinophagia and Lacibacter luteus sp. nov.
© 2020 The Authors
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2019-12-18
2025-04-24
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Lacibacter luteus sp. nov., isolated from a freshwater river
Int J Syst Evol Microbiol 70, 1404 (2020); https://doi.org/10.1099/ijsem.0.003926
/content/journal/ijsem/10.1099/ijsem.0.003926
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