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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 5

Characterization of gen. nov., sp. nov., isolated from human faeces, and proposal for the reclassification of within the genus Free

Abstract

A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium, designated strain ZHW00191, was isolated from human faeces and characterized by using a polyphasic taxonomic approach. Growth occurred at 25–45 °C (optimum, 37–42 °C), at pH 5.5–10.0 (optimum, pH 6.5–7.0) and with 0–2 % (w/v) NaCl (optimum, 0 %). The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid. The dominant cellular fatty acids (>10 %) of strain ZHW00191 were C, C 9 and Cω6,9. Its polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids and ten unidentified glycolipids. Respiratory quinones were not detected. The cell-wall peptidoglycan contained -2,6-diaminopimelic acid, and the whole-cell sugars were ribose and glucose. The genomic DNA G+C content was 32.8 mol%. Analysis of the 16S rRNA gene sequence indicated that ZHW00191 was most closely related to TO-931 (95.3 % similarity). Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95–96% and 70 % for species delineation, respectively. Based on phenotypic, chemotaxonomic and genetic studies, a novel genus, gen. nov., is proposed. The novel isolate ZHW00191 (=JCM 33482=GDMCC 1.1530) is proposed as the type strain of the type species gen. nov., sp. nov. of the proposed new genus. Furthermore, it is proposed that be transferred to this novel genus, as comb. nov.

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Keyword(s): genome analysis , Peptacetobacter hiranonis comb. nov. , Peptacetobacter hominis gen. nov., sp. nov and polyphasic taxonomy
Funding
This study was supported by the:
  • State Key Laboratory for Diagnosis and Treatment of Infectious Diseases (CN) (Award 2017ZX10103011006)
    • Principle Award Recipient: Hong-Wei Zhou
  • National Natural Science Foundation of China (Award NSFC31570497)
    • Principle Award Recipient: Hong-Wei Zhou
© 2020 The Authors
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2024-04-19
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Characterization of Peptacetobacter hominis gen. nov., sp. nov., isolated from human faeces, and proposal for the reclassification of Clostridium hiranonis within the genus Peptacetobacter
Int J Syst Evol Microbiol 70, 2988 (2020); https://doi.org/10.1099/ijsem.0.003925
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