Fourteen novel lipomycetaceous yeast species isolated from soil in Japan and transfer of to the genus based on ascospore production phenotype Free

Abstract

Fourteen novel lipomycetaceous yeasts species were isolated from soil samples collected from the Hokkaido, Chiba and Okinawa prefectures of Japan. Phylogenetic analyses of the D1/D2 domains of the large subunit rRNAs and translation elongation factor 1 alpha genes () revealed that five strains of two species from the soil in Furano-shi, Hokkaido were related to and 29 strains representing 12 species from soils in Kamogawa-shi, Chiba and Iriomote Island, Okinawa were in the clade. The two species of form globose or ellipsoid ascospores in their sac-like ascus and pseudohyphae. Furthermore, these species produce ascospores in their pseudohyphae and do not produce an acicular ascus, which is common among the three species including . Therefore, we propose transferring to the genus and amending . Two novel species were described and included in the genus : sp. nov. (holotype NBRC 111233; MycoBank no. MB 829051) and sp. nov. (holotype NBRC 112965; MycoBank no. MB 829053). The 12 species in the clade produce neither ascospores nor pseudohyphae and have different characteristics in assimilating several carbon sources from each other. Thus, we propose that the novel species of be classified as forma asexualis (f.a.). From Kamogawa-shi, Chiba (19 strains representing five species): f.a., sp. nov. (holotype NBRC 111411; MycoBank no. MB 829034), f.a., sp. nov. (holotype NBRC 111424; MycoBank no. MB 829035), f.a., sp. nov. (holotype NBRC 111413; MycoBank no. MB 829036), f.a., sp. nov. (holotype NBRC 112967; MycoBank no. MB 829037), f.a., sp. nov. (holotype NBRC 111420; MycoBank no. MB 829041). From Iriomote island, Okinawa (10 strains representing seven species): f.a., sp. nov. (holotype NBRC 112966; MycoBank no. MB 829042), f.a., sp. nov. (holotype NBRC 110433; MycoBank no. MB 829050), f.a., sp. nov. (holotype NBRC 110436; MycoBank no. MB 829045), f.a., sp. nov. (holotype NBRC 110435; MycoBank no. MB 829046), f.a., sp. nov. (holotype NBRC 110440; MycoBank no. MB 829047), f.a., sp. nov. (holotype NBRC 110434; MycoBank no. MB 829048), f.a., sp. nov. (holotype NBRC 110439; MycoBank no. MB 829049).

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2020-01-16
2024-03-28
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References

  1. Kurtzman CP, Albertyn J, Basehoar-Powers E. Multigene phylogenetic analysis of the Lipomycetaceae and the proposed transfer of Zygozyma species to Lipomyces and Babjevia anomala to Dipodascopsis . FEMS Yeast Res 2007; 7:1027–1034 [View Article]
    [Google Scholar]
  2. Starkey RL. Lipid production by a soil yeast. J Bacteriol 1946; 51:33–50
    [Google Scholar]
  3. Lodder J, Kreger-van Rij NJW. Genus Lipomyces . In Lodder J, Kreger-van Rij NJW. (editors) The Yeasts, A Taxonomic Study Amsterdam: North-Holland Publishing Company; 1952 pp 669–700
    [Google Scholar]
  4. Smith MT, Kurtzman CP. Lipomyces Lodder & Kreger-van Rij (1952). In Kurtzman CP, Fell JW, Boekout T. (editors) The Yeasts, A Taxonomic Study vol.2, 5th ed.. Amsterdam: Elsevier; 2011 pp 545–560
    [Google Scholar]
  5. Naganuma T, Uzuka Y, Tanaka K. Physiological factors affecting total cell number and lipid content of the yeast, Lipomyces starkeyi . J Gen Appl Microbiol 1985; 31:29–37 [View Article]
    [Google Scholar]
  6. Naganuma T, Uzuka Y, Tanaka K. Using inorganic elements to control cell growth and lipid accumulation in Lipomyces starkeyi . J Gen Appl Microbiol 1986; 32:417–424 [View Article]
    [Google Scholar]
  7. Zhao X, Kong X, Hua Y, Feng B, Zhao Zongbao (Kent). Medium optimization for lipid production through co-fermentation of glucose and xylose by the oleaginous yeastLipomyces starkeyi . Eur. J. Lipid Sci. Technol. 2008; 110:405–412 [View Article]
    [Google Scholar]
  8. Meng X, Yang J, Xu X, Zhang L, Nie Q et al. Biodiesel production from oleaginous microorganisms. Renewable Energy 2009; 34:1–5 [View Article]
    [Google Scholar]
  9. Oguri E, Masaki K, Naganuma T, Iefuji H. Phylogenetic and biochemical characterization of the oil-producing yeast Lipomyces starkeyi . Antonie van Leeuwenhoek 2012; 101:359–368 [View Article]
    [Google Scholar]
  10. Smith MT, de Hoog GS. Dipodascopsis Batra & P. Millner emend. Kurtzman, Albertyn & Basehoar-Powers (2007). In Kurtzman CP, Fell JW, Boekout T. (editors) The Yeasts, A Taxonomic Study vol.2, 5th ed.. Amsterdam: Elsevier; 2011 pp 379–384
    [Google Scholar]
  11. Kurtzman CP, Smith MT. Myxozyma van der Walt, Weijiman & von Arx (1981). In Kurtzman CP, Fell JW, Boekout T. (editors) The Yeasts, A Taxonomic Study vol.2, 5th ed.. Amsterdam: Elsevier; 2011 pp 1303–1312
    [Google Scholar]
  12. McNeill J, Barrie FR, Buck WR, Demoulin V, Greuter W et al. International Code of Nomenclature for algae, fungi, and plants (Melbourne Code Königstein: Koeltz Scientific; 2011
    [Google Scholar]
  13. Thanh VN. Lipomyces orientalis sp. nov., a yeast species isolated from soil in Vietnam. Int J Syst Evol Microbiol 2006; 56:2009–2013 [View Article]
    [Google Scholar]
  14. O’Donnell K. Fusarium and its near relatives. In Reynolds DR, Taylor JW. (editors) The fungal holomorph: mitotic meiotic and pleomorphic speciation in fungal systematics Wallingford: CAB International; 1993 pp 225–233
    [Google Scholar]
  15. Kurtzman C, Robnett C. Phylogenetic relationships among yeasts of the 'Saccharomyces complex' determined from multigene sequence analyses. FEMS Yeast Res 2003; 3:417–432 [View Article]
    [Google Scholar]
  16. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications New York: Academic Press, Inc; 1990 pp 315–322
    [Google Scholar]
  17. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425
    [Google Scholar]
  18. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article]
    [Google Scholar]
  19. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article]
    [Google Scholar]
  20. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article]
    [Google Scholar]
  21. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
    [Google Scholar]
  22. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekout T. (editors) The Yeasts, A Taxonomic Study vol. 1, 5th ed. Amsterdam: Elsevier; 2011 pp 87–110
    [Google Scholar]
  23. Yamazaki A, Kawasaki H. Lipomyces chichibuensis sp. nov., isolated in Japan, and reidentification of the type strains of Lipomyces kononenkoae and Lipomyces spencermartinsiae . Int J Syst Evol Microbiol 2014; 64:2566–2572 [View Article]
    [Google Scholar]
  24. Yamazaki A, Kanti A, Kawasaki H. Three novel lipomycetaceous yeasts, Lipomyces maratuensis sp. nov., Lipomyces tropicalis sp. nov., and Lipomyces kalimantanensis f.a., sp. nov. isolated from soil from the Maratua and Kalimantan Islands, Indonesia. Mycoscience 2017; 58:413–423 [View Article]
    [Google Scholar]
  25. Yamazaki A, Yanagiba M, Naganuma T. Two novel Lipomycetaceous yeast species, Lipomyces okinawensis sp. nov. and Lipomyces yamanashiensis FA, sp. nov., isolated from soil in the Okinawa and Yamanashi prefectures, Japan. Int J Syst Evol Microbiol 2017; 67:2941–2946
    [Google Scholar]
  26. Holm C, Meeks-Wagner DW, Fangman WL, Botstein D. A rapid, efficient method for isolating DNA from yeast. Gene 1986; 42:169–173 [View Article]
    [Google Scholar]
  27. Lachance MA. In defense of yeast sexual life cycles: the forma asexualis – an informal proposal. Yeast Newsletter 2012; 61:24–25
    [Google Scholar]
  28. Babjeva IP, Gorin SE. Lipomyces anomalus sp. nov. Antonie van Leeuwenhoek 1975; 41:185–191 [View Article]
    [Google Scholar]
  29. Smith MT, van der Walt JP, Batenburg-van der Vegte WH. Babjevia gen. nov. — a new genus of the Lipomycetaceae. Antonie van Leeuwenhoek 1995; 67:177–179 [View Article]
    [Google Scholar]
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