Fourteen novel lipomycetaceous yeast species isolated from soil in Japan and transfer of Dipodascopsis anomala to the genus Babjevia based on ascospore production phenotype
Fourteen novel lipomycetaceous yeasts species were isolated from soil samples collected from the Hokkaido, Chiba and Okinawa prefectures of Japan. Phylogenetic analyses of the D1/D2 domains of the large subunit rRNAs and translation elongation factor 1 alpha genes (TEF1-α) revealed that five strains of two species from the soil in Furano-shi, Hokkaido were related to Dipodascopsis anomala and 29 strains representing 12 species from soils in Kamogawa-shi, Chiba and Iriomote Island, Okinawa were in the Myxozyma clade. The two species of Dipodascopsis form globose or ellipsoid ascospores in their sac-like ascus and pseudohyphae. Furthermore, these species produce ascospores in their pseudohyphae and do not produce an acicular ascus, which is common among the three species including D. anomala. Therefore, we propose transferring D. anomala to the genus Babjevia and amending Babjevia. Two novel species were described and included in the genus Babjevia: Babjevia hyphoforaminiformans sp. nov. (holotype NBRC 111233; MycoBank no. MB 829051) and Babjevia hyphasca sp. nov. (holotype NBRC 112965; MycoBank no. MB 829053). The 12 species in the Myxozyma clade produce neither ascospores nor pseudohyphae and have different characteristics in assimilating several carbon sources from each other. Thus, we propose that the novel species of Lipomyces be classified as forma asexualis (f.a.). From Kamogawa-shi, Chiba (19 strains representing five species): Lipomyces melibiosiraffinosiphilus f.a., sp. nov. (holotype NBRC 111411; MycoBank no. MB 829034), Lipomyces kiyosumicus f.a., sp. nov. (holotype NBRC 111424; MycoBank no. MB 829035), Lipomyces chibensis f.a., sp. nov. (holotype NBRC 111413; MycoBank no. MB 829036), Lipomyces kamogawensis f.a., sp. nov. (holotype NBRC 112967; MycoBank no. MB 829037), Lipomyces amatsuensis f.a., sp. nov. (holotype NBRC 111420; MycoBank no. MB 829041). From Iriomote island, Okinawa (10 strains representing seven species): Lipomyces taketomicus f.a., sp. nov. (holotype NBRC 112966; MycoBank no. MB 829042), Lipomyces yaeyamensis f.a., sp. nov. (holotype NBRC 110433; MycoBank no. MB 829050), Lipomyces iriomotensis f.a., sp. nov. (holotype NBRC 110436; MycoBank no. MB 829045), Lipomyces haiminakanus f.a., sp. nov. (holotype NBRC 110435; MycoBank no. MB 829046), Lipomyces komiensis f.a., sp. nov. (holotype NBRC 110440; MycoBank no. MB 829047), Lipomyces nakamensis f.a., sp. nov. (holotype NBRC 110434; MycoBank no. MB 829048), Lipomyces sakishimensis f.a., sp. nov. (holotype NBRC 110439; MycoBank no. MB 829049).
KurtzmanCP,
AlbertynJ,
Basehoar-PowersE.
Multigene phylogenetic analysis of the Lipomycetaceae and the proposed transfer of Zygozyma species to Lipomyces and Babjevia anomala to Dipodascopsis
. FEMS Yeast Res2007; 7:1027–1034 [View Article]
LodderJ,
Kreger-van RijNJW.
Genus Lipomyces
. In
LodderJ,
Kreger-van RijNJW.
(editors) The Yeasts, A Taxonomic Study Amsterdam: North-Holland Publishing Company; 1952 pp 669–700
NaganumaT,
UzukaY,
TanakaK.
Physiological factors affecting total cell number and lipid content of the yeast, Lipomyces starkeyi
. J Gen Appl Microbiol1985; 31:29–37 [View Article]
NaganumaT,
UzukaY,
TanakaK.
Using inorganic elements to control cell growth and lipid accumulation in Lipomyces starkeyi
. J Gen Appl Microbiol1986; 32:417–424 [View Article]
ZhaoX,
KongX,
HuaY,
FengB,
ZhaoZongbao (Kent).
Medium optimization for lipid production through co-fermentation of glucose and xylose by the oleaginous yeastLipomyces starkeyi
. Eur. J. Lipid Sci. Technol.2008; 110:405–412 [View Article]
KurtzmanCP,
SmithMT.
Myxozyma van der Walt, Weijiman & von Arx (1981). In
KurtzmanCP,
FellJW,
BoekoutT.
(editors) The Yeasts, A Taxonomic Studyvol.2, 5th ed.. Amsterdam: Elsevier; 2011 pp 1303–1312
O’DonnellK.
Fusarium and its near relatives. In
ReynoldsDR,
TaylorJW.
(editors) The fungal holomorph: mitotic meiotic and pleomorphic speciation in fungal systematics Wallingford: CAB International; 1993 pp 225–233
WhiteTJ,
BrunsT,
LeeS,
TaylorJW.
Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In
InnisMA,
GelfandDH,
SninskyJJ,
WhiteTJ.
(editors) PCR Protocols: A Guide to Methods and Applications New York: Academic Press, Inc; 1990 pp 315–322
KimuraM.
A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol1980; 16:111–120 [View Article]
YamazakiA,
KawasakiH.
Lipomyces chichibuensis sp. nov., isolated in Japan, and reidentification of the type strains of Lipomyces kononenkoae and Lipomyces spencermartinsiae
. Int J Syst Evol Microbiol2014; 64:2566–2572 [View Article]
YamazakiA,
KantiA,
KawasakiH.
Three novel lipomycetaceous yeasts, Lipomyces maratuensis sp. nov., Lipomyces tropicalis sp. nov., and Lipomyces kalimantanensis f.a., sp. nov. isolated from soil from the Maratua and Kalimantan Islands, Indonesia. Mycoscience2017; 58:413–423 [View Article]
YamazakiA,
YanagibaM,
NaganumaT.
Two novel Lipomycetaceous yeast species, Lipomyces okinawensis sp. nov. and Lipomyces yamanashiensis FA, sp. nov., isolated from soil in the Okinawa and Yamanashi prefectures, Japan. Int J Syst Evol Microbiol2017; 67:2941–2946
SmithMT,
van der WaltJP,
Batenburg-van der VegteWH.
Babjevia gen. nov. — a new genus of the Lipomycetaceae. Antonie van Leeuwenhoek1995; 67:177–179 [View Article]
Fourteen novel lipomycetaceous yeast species isolated from soil in Japan and transfer of Dipodascopsis anomala to the genus Babjevia based on ascospore production phenotype