1887

Abstract

A novel actinobacterial strain, designated 18, was isolated from a steppe soil sample collected in Buryatia, Russia and subjected to polyphasic taxonomic characterization. The strain was aerobic and Gram-stain-positive. The 16S rRNA gene sequence of strain 18 exhibited highest similarity to CPCC 204357 (97.2 %). Results of phylogenetic analysis showed that strain 18 formed a distinct branch clearly affiliated to the genus . Whole-cell hydrolysates of the isolate contained -diaminopimelic acid as the cell-wall diamino acid. The whole-cell sugar profile was found to contain galactose, glucose, ribose and xylose. MK-10(H) and MK-11(H) were the predominant menaquinones. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, five unidentified phospholipids and four unidentified polar lipids. The major fatty acids identified were anteiso-C and iso-C. The G+C content of the genomic DNA of strain 18 was 68.0 mol% (draft genome sequence). Based on its phylogenetic, phenotypic and chemotaxonomic features, strain 18 was considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is 18 (=JCM 33362=CGMCC 4.7610).

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/content/journal/ijsem/10.1099/ijsem.0.003923
2019-12-06
2020-01-24
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