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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 2

sp. nov., a novel actinobacterium isolated from soil Free

Abstract

A novel actinobacterium, designated strain NEAU-GS14, was isolated from soil of a flower bed in a residential area in Sanya, Hainan Province, China, and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics of the strain coincided with members of the genus Herbidospora. The 16S rRNA gene sequence analysis showed that strain NEAU-GS14 belongs to the genus and was most closely related to JCM 18061 (98.8 %), other type strains of species of the genus were found to be less than 98.7 %. Phylogenetic analysis using the 16S rRNA gene sequences showed that the strain formed a cluster with JCM 18061. Cell wall contained -diaminopimelic acid as the major diamino acid and the whole-cell hydrolysates were glucose, madurose and ribose. The major polar lipids were diphosphatidylglycerol, hydroxyphosphatidylethanolamine, phosphatidylethanolamine phosphatidylmethylethanolamine, phosphoglycolipids and two phosphatidylinositol mannosides. The predominant menaquinone was MK-10(H). Major fatty acids were 10-methly C and C, these chemotaxonomic data supported the affiliation of strain NEAU-GS14 to the genus . The DNA G+C content was 70.6 mol%. Furthermore, the strain could be clearly distinguished by digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values and some phenotypic characteristics. Therefore, it is proposed that strain NEAU-GS14 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-GS14 (=CCTCC AA 2018040=JCM 33459).

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  • Accepted:
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  • Published Online:
Keyword(s): 16S rRNA gene , Herbidospora galbida sp. nov and polyphasic taxonomy
Funding
This study was supported by the:
  • he National Natural Science Foundation of China (Award 31872037)
    • Principle Award Recipient: Wensheng Xiang
© 2020 The Authors
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2019-12-12
2024-04-16
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Herbidospora galbida sp. nov., a novel actinobacterium isolated from soil
Int J Syst Evol Microbiol 70, 1364 (2020); https://doi.org/10.1099/ijsem.0.003922
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