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Abstract

Two strains of anaerobic, coccoid, saccharolytic, Gram-stain-negative bacteria were isolated from samples of anoxic hypersaline sediments of evaporation ponds in Tavira (Portugal) and Mallorca (Spain). Both isolates were moderately halophilic, neutrophilic and had a temperature optimum at 37 °C. The highest 16S rRNA gene sequence identity values were found with members of the genus (84.9–88.2 %) within the order class . The strain SM-Chi-D1 could be assigned to the family , while phylogenetic analyses based on 16S rRNA gene sequences and genomic data indicate that strain ST-NAGAB-D1 is both a member of a novel genus and a novel family. SM-Chi-D1 could be distinguished from other cultured members of the mainly by the stimulatory effect of sulfur on growth, lack of ethanol production during fermentation and several differences in the cellular fatty acids and polar lipids patterns. Main differential characteristics of ST-NAGAB-D1 were a polytrichous flagellation, the absence of branched chain fatty acids and presence of large proportions of the unsaturated cellular fatty acids C 9 and C 11. On the basis of genomic, chemotaxonomic, biochemical and physiological data, we propose the novel species and genera gen. nov., sp. nov., and gen. nov., sp. nov., represented by the type strains ST-NAGAB-D1 (=DSM 103484=JCM 31926=KCTC 15600) and SM-Chi-D1 (=DSM 100118=JCM 31927=KCTC 15601), respectively. In addition, we propose the novel family fam. nov. to accommodate the genus .

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2019-12-05
2024-04-25
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References

  1. Spring S, Bunk B, Spröer C, Rohde M, Klenk HP. Genome biology of a novel lineage of Planctomycetes widespread in anoxic aquatic environments. Environ Microbiol 2018; 20:2438–2455 [View Article]
    [Google Scholar]
  2. Bryant MP. Commentary on the Hungate technique for culture of anaerobic bacteria. Am J Clin Nutr 1972; 25:1324–1328 [View Article]
    [Google Scholar]
  3. Yarza P, Yilmaz P, Pruesse E, Glöckner FO, Ludwig W et al. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol 2014; 12:635–645 [View Article]
    [Google Scholar]
  4. Parks DH, Chuvochina M, Waite DW, Rinke C, Skarshewski A et al. A standardized bacterial taxonomy based on genome phylogeny substantially revises the tree of life. Nat Biotechnol 2018; 36:996–1004 [View Article]
    [Google Scholar]
  5. Nguyen L-T, Schmidt HA, von Haeseler A, Minh BQ. IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol Biol Evol 2015; 32:268–274 [View Article]
    [Google Scholar]
  6. Kalyaanamoorthy S, Minh BQ, Wong TKF, von Haeseler A, Jermiin LS. ModelFinder: fast model selection for accurate phylogenetic estimates. Nat Methods 2017; 14:587–589 [View Article]
    [Google Scholar]
  7. Hoang DT, Chernomor O, von Haeseler A, Minh BQ, Vinh LS. UFBoot2: improving the ultrafast bootstrap approximation. Mol Biol Evol 2018; 35:518–522 [View Article]
    [Google Scholar]
  8. Fardeau ML, Ollivier B, Patel BK, Magot M, Thomas P et al. Thermotoga hypogea sp. nov., a xylanolytic, thermophilic bacterium from an oil-producing well. Int J Syst Bacteriol 1997; 47:1013–1019 [View Article]
    [Google Scholar]
  9. Cord-Ruwisch R. A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. J Microbiol Methods 1985; 4:33–36 [View Article]
    [Google Scholar]
  10. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990; 13:128–130 [View Article]
    [Google Scholar]
  11. Tindall BJ. Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 1990; 66:199–202 [View Article]
    [Google Scholar]
  12. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy C, Beveridge T, Breznak J, Marzluf G, Schmidt T. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: American Society of Microbiology; 2007 pp 330–393
    [Google Scholar]
  13. Kuykendall LD, Roy MA, O'NEILL JJ, Devine TE. Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 1988; 38:358–361 [View Article]
    [Google Scholar]
  14. Miller LT. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 1982; 16:584–586
    [Google Scholar]
  15. Elshahed MS, Youssef NH, Luo Q, Najar FZ, Roe BA et al. Phylogenetic and metabolic diversity of Planctomycetes from anaerobic, sulfide- and sulfur-rich Zodletone Spring, Oklahoma. Appl Environ Microbiol 2007; 73:4707–4716 [View Article]
    [Google Scholar]
  16. Slobodkina GB, Kovaleva OL, Miroshnichenko ML, Slobodkin AI, Kolganova TV et al. Thermogutta terrifontis gen. nov., sp. nov. and Thermogutta hypogea sp. nov., thermophilic anaerobic representatives of the phylum Planctomycetes . Int J Syst Evol Microbiol 2015; 65:760–765 [View Article]
    [Google Scholar]
  17. Slobodkina GB, Panteleeva AN, Beskorovaynaya DA, Bonch-Osmolovskaya EA, Slobodkin AI. Thermostilla marina gen. nov., sp. nov., a thermophilic, facultatively anaerobic planctomycete isolated from a shallow submarine hydrothermal vent. Int J Syst Evol Microbiol 2016; 66:633–638 [View Article]
    [Google Scholar]
  18. Rodriguez-R LM, Konstantinidis KT. The enveomics collection : a toolbox for specialized analyses of microbial genomes and metagenomes. Peer J Prepr 2016; 4:e1900v1
    [Google Scholar]
  19. Konstantinidis KT, Rosselló-Móra R, Amann R. Uncultivated microbes in need of their own taxonomy. ISME J 2017; 11:2399–2406 [View Article]
    [Google Scholar]
  20. Liu R, Ochman H. Stepwise formation of the bacterial flagellar system. Proc Natl Acad Sci USA 2007; 104:7116–7121 [View Article]
    [Google Scholar]
  21. Fuerst JA, Sagulenko E. Beyond the bacterium: Planctomycetes challenge our concepts of microbial structure and function. Nat Rev Microbiol 2011; 9:403–413 [View Article]
    [Google Scholar]
  22. Boedeker C, Schüler M, Reintjes G, Jeske O, van Teeseling MCF et al. Determining the bacterial cell biology of Planctomycetes. Nat Commun 2017; 8:14853 [View Article]
    [Google Scholar]
  23. Zhang Y-M, Rock CO. Thematic review series: glycerolipids. acyltransferases in bacterial glycerophospholipid synthesis. J Lipid Res 2008; 49:1867–1874 [View Article]
    [Google Scholar]
  24. Wang H, Cronan JE. Functional replacement of the FabA and FabB proteins of Escherichia coli fatty acid synthesis by Enterococcus faecalis FabZ and FabF homologues. J Biol Chem 2004; 279:34489–34495 [View Article]
    [Google Scholar]
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