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Abstract

A Gram-staining-negative, aerobic, curved rod-shaped and thermophilic bacterial strain, designated YIM 72297, was isolated from a sediment sample collected from a hot spring in Tengchong county, Yunnan province, south-west China. Growth was observed at pH 5.0–9.0 with an optimum of pH 7.0–7.5, and at 45–60 °C with an optimum of 55 °C. Positive for catalase and oxidase. The 16S rRNA gene sequence comparison indicated that strain YIM 72297 was most closely related to DSM 17972 (96.9 %) and showed <91 % sequence similarities to members of the order . Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM 72297 formed a distinct lineage within the genus , and revealed that the genus formed a novel family-level clade in the order . The ANI and the dDNA-DNA hybridization estimate values between strains YIM 72297 and DSM 17972 were 70.8 and 20.1 %, respectively. Strain YIM 72297 contained Q-10 as the predominant ubiquinone. The major fatty acids (>5 %) were summed C (35.8 %), summed feature 8 (30.1 %), C (12.6 %), C 2OH (5.6 %) and C 2OH (5.4 %). The polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, diphosphosphatidylglycerol and phosphatidylglycerol in addition to two unidentified aminolipids. The DNA G+C content of YIM 72297 was 70.8 mol% (draft genome). On the basis of the polyphasic taxonomic evidence presented in this study, strain YIM 72297 should be classified as representing a novel species of the genus , for which the name sp. nov. is proposed, with the type strain YIM 72297 (=CCTCC AB 2017169=KCTC 62323). In addition, a novel family, fam. nov., is proposed to accommodate the genus .

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2019-12-05
2020-01-24
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