1887

Abstract

A phototrophic bacterium, designated as strain JA983, was isolated from a freshwater pond in Gujarat, India. The strain was yellowish brown, catalase- and oxidase-positive, rod-to-oval shaped, Gram-stain-negative and motile. Growth was observed at 20–35 °C. NaCl was not required for optimum growth and up to 5 % was tolerated. Growth was observed at pH 6.0–8.0, with an optimum at pH 7.0. An unidentified glycolipid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified aminolipids (AL1, AL2) and two unidentified lipids (L1 and L2) are the polar lipids of JA983. Q10 is the only quinone. C ω7/C ω6 is the major fatty acid. JA983 showed highest 16S rRNA gene sequence similarity with the type strains of (98.99%), (98.99 %), (98.99 %) and other members of the genus with less than 98.7 % similarity. In a 16S rRNA gene sequence-based phylogenetic tree, JA983 formed a different sub-clade with its nearest phylogenetic members of genus . Phenotypic, chemotaxonomic, phylogenetic and genomic [average nucleotide identity (ANI) and digital DNA–DNA hybridization (DDH) differences indicated that JA983 is significantly different from other species of the genus and thus represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is JA983 (=KCTC 15782=NBRC 113843).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003913
2019-12-05
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/70/2/1294.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003913&mimeType=html&fmt=ahah

References

  1. Imhoff JF. Genus Rhodobacter . In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey’s Manual of Systematic Bacteriology 2, part C, 2nd ed. New York: Springer; 2005 pp 161–167
    [Google Scholar]
  2. Girija KR, Sasikala C, Ramana CV, Sproer C, Takaichi S et al. Rhodobacter johrii sp. nov., an endospore-producing cryptic species isolated from semi-arid tropical soils. Int J Syst Evol Microbiol 2010; 60:2099–2107 [View Article]
    [Google Scholar]
  3. Suresh G, Sailaja B, Ashif A, Dave BP, Sasikala C et al. Description of Rhodobacter azollae sp. nov. and Rhodobacter lacus sp. nov. Int J Syst Evol Microbiol 2017; 67:3289–3295 [View Article]
    [Google Scholar]
  4. Gandham S, Lodha T, Chintalapati S, Chintalapati VR. Rhodobacter alkalitolerans sp. nov., isolated from an alkaline brown pond. Arch Microbiol 2018; 200:1487–1492 [View Article]
    [Google Scholar]
  5. Raj PS, Ramaprasad EVV, Vaseef S, Sasikala C, Ramana CV. Rhodobacter viridis sp. nov., a phototrophic bacterium isolated from mud of a stream. Int J Syst Evol Microbiol 2013; 63:181–186 [View Article]
    [Google Scholar]
  6. Hanada S, Matsuura K, Nakamura K, Takaichi S. Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. Int J Syst Evol Microbiol 2002; 52:187–193 [View Article]
    [Google Scholar]
  7. Trüper HG, Pfennig N. Characterization and Identification of the Anoxygenic Phototrophic Bacteria. In Starr MP, Stolp H, Trüper HG. (editors) The Prokaryotes: a Handbook on Habitats, Isolation, and Identification of Bacteria 1 Springer: New York; 1981 pp 298–312
    [Google Scholar]
  8. Oren A. Characterization of pigments of prokaryotes and their use in taxonomy and classification. Method Microbiol 2011; 36:261–282
    [Google Scholar]
  9. Nobel PS. Photochemistry of photosynthesis. Physicochemical and Environmental Plant Physiology 4 2009 pp 228–275
    [Google Scholar]
  10. Ramaprasad EVV, Sasikala Ch, Ramana ChV. Neurosporene is the major carotenoid accumulated by Rhodobacter viridis JA737. Biotechnol Lett 2013; 35:1093–1097 [View Article]
    [Google Scholar]
  11. Imhoff JF. Quinones of phototrophic purple bacteria. FEMS Microbiol Lett 1984; 25:85–89 [View Article]
    [Google Scholar]
  12. Hiraishi A, Hoshino Y. Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. J Gen Appl Microbiol 1984; 30:435–448 [View Article]
    [Google Scholar]
  13. Hiraishi A, Hoshino Y, Kitamura H. Isoprenoid quinone composition in the classification of Rhodospirillaceae . J Gen Appl Microbiol 1984; 30:197–210 [View Article]
    [Google Scholar]
  14. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  15. Kates M. Techniques of Lipidology. Isolation, analysis and identification of lipids. In Burdon RH, van Knippenberg PH. (editors) Laboratory Techniques in Biochemistry and Molecular Biology 3, part 2 Elsevier: Amsterdam; 1986 pp 100–112
    [Google Scholar]
  16. Tindall BJ. Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 1990; 66:199–202 [View Article]
    [Google Scholar]
  17. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990; 13:128–130 [View Article]
    [Google Scholar]
  18. Oren A, Duker S, Ritter S. The polar lipid composition of Walsby's square bacterium. FEMS Microbiol Lett 1996; 138:135–140 [View Article]
    [Google Scholar]
  19. Kates M. Techniques in lipidology. Laboratory Techniques in Biochemistry and Molecular Biology part 2 American Elsevier Publishing Company 3; 1972 pp 355–356
    [Google Scholar]
  20. Divyasree B, Suresh G, Sasikala C, Ramana CV. Chryseobacterium salipaludis sp. nov., isolated at a wild ass sanctuary. Int J Syst Evol Microbiol 2018; 68:542–546 [View Article]
    [Google Scholar]
  21. Yoon SH, SM H, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617
    [Google Scholar]
  22. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article]
    [Google Scholar]
  23. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article]
    [Google Scholar]
  24. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article]
    [Google Scholar]
  25. Auch AF, Klenk H-P, Göker M. Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. Stand Genomic Sci 2010; 2:142–148 [View Article]
    [Google Scholar]
  26. Rosselló-Móra R, Amann R. Past and future species definitions for bacteria and Archaea . Syst Appl Microbiol 2015; 38:209–216 [View Article]
    [Google Scholar]
  27. Lee I, Ouk Kim Y, Park S-C, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article]
    [Google Scholar]
  28. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article]
    [Google Scholar]
  29. Suresh G, Lodha TD, Indu B, Sasikala C, Ramana CV. Taxogenomics resolves conflict in the genus Rhodobacter: a two and half decades pending thought to reclassify the genus Rhodobacter . Front Microbiol 2019; 10:2480 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003913
Loading
/content/journal/ijsem/10.1099/ijsem.0.003913
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error