@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003912, author = "Dong, Kui and Yang, Jing and Lu, Shan and Pu, Ji and Lai, Xin-He and Jin, Dong and Li, Junqin and Zhang, Gui and Wang, Xiaoxia and Zhang, Sihui and Lei, Wenjing and Ren, Zhihong and Wu, Xiaomin and Huang, Ying and Wang, Suping and Xu, Jianguo", title = "Microbacterium wangchenii sp. nov., isolated from faeces of Tibetan gazelles (Procapra picticaudata) on the Qinghai-Tibet Plateau", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "2", pages = "1307-1314", doi = "https://doi.org/10.1099/ijsem.0.003912", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003912", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Qinghai-Tibet Plateau", keywords = "Microbacterium", keywords = "Tibetan gazelle", keywords = "novel species", abstract = "Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China. The 16S rRNA gene sequences of the strains showed the highest identity to Microbacterium saccharophilum K-1T (98.0 and 97.9 % similarity, respectively). The phylogenetic analysis based on 16S rRNA gene sequences revealed that dk512T and dk508 were members of the genus Microbacterium , and most closely related to strains Microbacterium mitrae M4-8T and Microbacterium hatanonis FCC-01T. The strains grew optimally on brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl. The genome of type strain dk512T was 3.8 Mb with a G+C content of 70.6 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain dk512T and previously characterized Microbacterium species were <95 and <70 %, respectively. In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids. The major cell-wall sugars were rhamnose, ribose and galactose. Alanine, glutamic acid, glycine and ornithine were in the cell-wall peptidoglycan. Based on phenotypic data and phylogenetic inference, these two strains represent a novel species of the genus Microbacterium , named here as Microbacterium wangchenii sp. nov, where dk512T is designated the type strain (=CGMCC 1.16590T=JCM 33494T=KCTC 49313T).", }