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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 2

gen. nov., sp. nov., a moderately thermophilic bacterium from a Chukotka hot spring (Arctic, Russia), representing a novel class, , which includes the previously uncultivated lineage OLB14 Free

Abstract

A novel aerobic moderately thermophilic bacterium, strain 3753O, was isolated from a Chukotka hot spring (Arctic, Russia) using the newly developed technology of laser engineering of microbial systems. Сells were regular short rods, 0.4×0.8–2.0 µm in size, with a monoderm-type envelope and a single flagellum. The temperature and pH ranges for growth were 42–60 °C and pH 6.5–8.5, the optima being 50–54 °C and pH 7.3. Strain 3753O grew chemoorganoheterotrophically on a number of carbohydrates or peptidic substrates and volatile fatty acids, and chemolithoautotrophically with siderite (FeCO) as the electron donor. The major cellular fatty acid was branched C. Phosphatidylethanolamine, phosphatidylglycerol and two unidentified phospholipids as well as two yellow carotenoid-type pigments were detected in the polar lipid extract. Strain 3753O was inhibited by chloramphenicol, polymyxin B, vancomycin, streptomycin, neomycin and kanamycin, but resistant to the action of novobiocin and ampicillin. The DNA G+C content was 69.9 mol%. The 16S rRNA gene as well as 51 conservative protein sequence-based phylogenetic analyses placed strain 3753O within the previously uncultivated lineage OLB14 in the phylum . Taking into account the phylogenetic position as well as phenotypic properties of the novel isolate, the novel genus and species gen. nov. sp. nov., within the fam. nov., the ord. nov. and the classis nov. are proposed. The type strain of is 3753O (=VKM B-3389=KTCT 72284).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Chloroflexi , Chukotka hot spring , laser bioprinting , LEMS , microbial dark matter and Tepidiforma
Funding
This study was supported by the:
  • the President of Russia (Award МК-3801.2018.4)
    • Principle Award Recipient: Andrei A. Novikov
  • Scholarship of the President of the Russian Federation (Award SP-2728.2019.4.)
    • Principle Award Recipient: Nikita V. Minaev
  • Ministry of Science and Higher Education within the State assignment FRC “Fundamentals of Biotechnology” RAS
  • Russian Foundation for Basic Research (Award 17-04-01578)
    • Principle Award Recipient: Not Applicable
© 2020 The Authors
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Tepidiforma bonchosmolovskayae gen. nov., sp. nov., a moderately thermophilic Chloroflexi bacterium from a Chukotka hot spring (Arctic, Russia), representing a novel class, Tepidiformia, which includes the previously uncultivated lineage OLB14
Int J Syst Evol Microbiol 70, 1192 (2020); https://doi.org/10.1099/ijsem.0.003902
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