1887

Abstract

One Gram-stain-positive, non-motile, non-spore-forming, catalase-negative, and coccobacilli-shaped strain, designated c10Ua161M, was isolated from a urine sample from a reproductive-age healthy woman. Comparative 16S rRNA gene sequence analysis indicated that strain c10Ua161M belonged to the genus . Phylogenetic analysis based on and gene sequences strongly supported a clade encompassing strains c10Ua161M and eight other strains from public databases, distinct from currently recognized species of the genus Average Nucleotide Identity (ANI) and Genome-to-Genome Distance Calculator (GGDC), showed 87.9 and 34.3 % identity to the closest relative , respectively. The major fatty acids of strain c10Ua161M were Cω (65.0%), C (17.8%), and summed feature 8 (10.2 %; comprising Cω7, and/or Cω6). The DNA G+C content of the strains is 34.2 mol%. On the basis of data presented here, strain c10Ua161M represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is c10Ua161M (=CECT 9755=DSM 108704).

Keyword(s): 16S rRNA , fatty acid , genome , pheS and rpoA
Funding
This study was supported by the:
  • Magdalena Ksiezarek , Fundação para a Ciência e a Tecnologia , (Award SFRH/BD/132497/2017)
  • João Botelho , Fundação para a Ciência e a Tecnologia , (Award SFRH/BD/104095/2014)
  • Sofia Salsinha , Fundação para a Ciência e a Tecnologia , (Award SFRH/BD/136857/2018)
  • Lígia Leão Pimentel , Fundação para a Ciência e a Tecnologia , (Award SFRH/BPD/119785/2016)
  • Not Applicable , Centro de Biotecnologia e Química Fina , (Award UID/Multi/50016/2013)
  • Not Applicable , Unidade de Ciências Biomoleculares Aplicadas , (Award UID/Multi/04378/2019)
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2020-01-17
2021-03-08
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