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Abstract
One Gram-stain-positive, non-motile, non-spore-forming, catalase-negative, and coccobacilli-shaped strain, designated c10Ua161MT, was isolated from a urine sample from a reproductive-age healthy woman. Comparative 16S rRNA gene sequence analysis indicated that strain c10Ua161MT belonged to the genus Lactobacillus . Phylogenetic analysis based on pheS and rpoA gene sequences strongly supported a clade encompassing strains c10Ua161MT and eight other strains from public databases, distinct from currently recognized species of the genus Lactobacillus. In silico Average Nucleotide Identity (ANI) and Genome-to-Genome Distance Calculator (GGDC), showed 87.9 and 34.3 % identity to the closest relative Lactobacillus jensenii , respectively. The major fatty acids of strain c10Ua161MT were C18 : 1ω9c (65.0%), C16 : 0 (17.8%), and summed feature 8 (10.2 %; comprising C18 : 1ω7c, and/or C18 : 1ω6c). The DNA G+C content of the strains is 34.2 mol%. On the basis of data presented here, strain c10Ua161MT represents a novel species of the genus Lactobacillus , for which the name Lactobacillus mulieris sp. nov. is proposed. The type strain is c10Ua161MT (=CECT 9755T=DSM 108704T).
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Funding
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Fundação para a Ciência e a Tecnologia
(Award SFRH/BD/132497/2017)
- Principle Award Recipient: Magdalena Ksiezarek
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Fundação para a Ciência e a Tecnologia
(Award SFRH/BD/104095/2014)
- Principle Award Recipient: João Botelho
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Fundação para a Ciência e a Tecnologia
(Award SFRH/BD/136857/2018)
- Principle Award Recipient: Sofia Salsinha
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Fundação para a Ciência e a Tecnologia
(Award SFRH/BPD/119785/2016)
- Principle Award Recipient: Lígia Leão Pimentel
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Centro de Biotecnologia e Química Fina
(Award UID/Multi/50016/2013)
- Principle Award Recipient: Not Applicable
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Unidade de Ciências Biomoleculares Aplicadas
(Award UID/Multi/04378/2019)
- Principle Award Recipient: Not Applicable