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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 2

sp. nov. isolated from Badain Jaran desert in China Free

Abstract

A Gram-stain-negative, non-motile, coccobacillus-shaped bacterium, designated CPCC 101021, was isolated from a sandy soil sample collected from Badain Jaran desert, China. Its 16S rRNA gene sequence was closely related to those of members of the genus showing high similarities with THG-N2.22 (98.0 %), KCTC 42542 (97.9 %), YW11 (97.9 %) and S1 (97.8 %). In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 101021 formed a distinct subclade with KCTC 42542 within the genus . Growth of the isolate occurred at 15–37 °C and pH 6.0–8.5, with optimal growth at 30 °C and pH 7.0. The major cellular fatty acids were Cω, summed feature 8 (Cωc/Cω), summed feature 3 (Cωc/Cω) and Cω. Q-10 was detected as the main component in the respiratory quinone system, with Q-9 as a minor component. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an unidentified phospholipid, an unidentified aminolipid and an unidentified glycolipid were found in the polar lipid profile. The genomic DNA G+C content was 68.7 mol%. The average nucleotide identity was 84.6 % when comparing the draft genome sequences of strain CPCC 101021 with KCTC 42542. On the basis of genotypic, chemotaxonomic and phenotypic characteristics, strain CPCC 101021 is proposed to represent a novel species of the genus with the name sp. nov. Strain CPCC 101021 (=J1A743=KCTC 62043) is the type strain of the species.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): 16S rRNA , genome , polyphasic and Roseomonas vastitatis
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31670010)
    • Principle Award Recipient: Yu-Qin Zhang
© 2020 The Authors
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2019-12-20
2024-04-19
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Roseomonas vastitatis sp. nov. isolated from Badain Jaran desert in China
Int J Syst Evol Microbiol 70, 1186 (2020); https://doi.org/10.1099/ijsem.0.003898
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