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Volume 70, Issue 2

sp. nov., an actinobacterium isolated from sand of the Taklamakan desert, and emended description of the genus Free

Abstract

A novel actinobacterium, designated strain 16Sb5-5, was isolated from a sand sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China. The strain was examined by a polyphasic approach to clarify its taxonomic position. Cells of the isolate were Gram-staining-positive, aerobic, non-motile and short-rod shaped. Strain 16Sb5-5 grew optimally at 37 °C, pH 7.0 and with 0‒2 % (w/v) NaCl. The cell-wall peptidoglycan was of the A3 type and contained alanine, glycine, glutamic acid and -diaminopimelic acid (-DAP). Ribose, arabinose and glucose were detected in the whole-cell hydrolysates. The predominant menaquinone was MK-9(H). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified phospholipid, three unidentified glycolipids and three unidentified lipids. The major whole-cell fatty acids were anteiso-C and iso-C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5 was closely related to CPCC 204711 (99.8 % similarity) and formed a robust clade with in the phylogenetic trees. genomic comparisons showed that strain 16Sb5-5 exhibited ANI values of 94.8–94.9 % and GGDC value of 59.5 % to CPCC 204711. The genomic G+C content was 73.3 mol%. On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5 could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 16Sb5-5 (=KCTC 49116=CGMCC 1.16553). The description of the genus has also been emended.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): actinobacteria , Desertihabitans brevis , Propionibacteriaceae , strain 16Sb5-5T and taxonomy
Funding
This study was supported by the:
  • National Natural Sciences Foundation of China (Award 81621064)
    • Principle Award Recipient: Cheng-Hang Sun
  • National Natural Sciences Foundation of China (Award 81321004)
    • Principle Award Recipient: Cheng-Hang Sun
  • National Natural Sciences Foundation of China (Award 81803411)
    • Principle Award Recipient: Shao-Wei Liu
  • CAMS innovation Fund for Medical Sciences (Award 2017-I2M-B and R-08)
  • the Drug Innovation Major Project of China (Award 2018ZX09711001-007-001)
    • Principle Award Recipient: Li-Yan Yu
© 2020 The Authors
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2019-11-26
2024-12-12
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Desertihabitans brevis sp. nov., an actinobacterium isolated from sand of the Taklamakan desert, and emended description of the genus Desertihabitans
Int J Syst Evol Microbiol 70, 1166 (2020); https://doi.org/10.1099/ijsem.0.003896
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