@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003894, author = "Shimizu, Yumi and Konno, Yusuke and Tomita, Yosuke", title = "Wickerhamomyces psychrolipolyticus f.a., sp. nov., a novel yeast species producing two kinds of lipases with activity at different temperatures", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "2", pages = "1158-1165", doi = "https://doi.org/10.1099/ijsem.0.003894", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003894", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "novel species", keywords = "psychrotrophic yeast", keywords = "subarctic climates", keywords = "Cold-active lipase", abstract = "Two yeast strains isolated from soil collected in Hokkaido, Japan, were found to secrete two extracellular lipases that exhibited activities at both 25 and 4 °C. Both strains could utilize olive oil, rapeseed oil, lard and fish oil as sole carbon sources. The similarity of the D1/D2 domain of the large subunit ribosomal RNA (LSU rRNA) sequence of these yeast strains to that of other yeasts in the GenBank database was very low (<96 %). The phylogenetic trees based on the LSU rRNA sequences and translation elongation factor-1-α (tef1-α) sequences indicated that both strains represented a member of the Wickerhamomyces /Candida clade. Sexual reproduction was not observed. The name Wickerhamomyces psychrolipolyticus f.a., sp. nov is proposed for this newly described yeast species producing cold-active lipases. This novel species is distinguishable from the type strains of other related species, Wickerhamomyces alni, Candida ulmi and Candida quercuum due to their abilities to grow at 4 to 30 °C, to produce lipase that is active also at 4 °C and to assimilate soluble starch.", }