1887

Abstract

Two yeast strains isolated from soil collected in Hokkaido, Japan, were found to secrete two extracellular lipases that exhibited activities at both 25 and 4 °C. Both strains could utilize olive oil, rapeseed oil, lard and fish oil as sole carbon sources. The similarity of the D1/D2 domain of the large subunit ribosomal RNA (LSU rRNA) sequence of these yeast strains to that of other yeasts in the GenBank database was very low (<96 %). The phylogenetic trees based on the LSU rRNA sequences and translation elongation factor-1-α () sequences indicated that both strains represented a member of the / clade. Sexual reproduction was not observed. The name f.a., sp. nov is proposed for this newly described yeast species producing cold-active lipases. This novel species is distinguishable from the type strains of other related species, , and due to their abilities to grow at 4 to 30 °C, to produce lipase that is active also at 4 °C and to assimilate soluble starch.

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/content/journal/ijsem/10.1099/ijsem.0.003894
2019-12-13
2020-01-24
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References

  1. Nagarajan S. New tools for exploring "old friends-microbial lipases". Appl Biochem Biotechnol 2012;168:1163–1196 [CrossRef]
    [Google Scholar]
  2. Neang PM, Subileau M, Perrier V, Dubreucq E. Homologous yeast lipases/acyltransferases exhibit remarkable cold-active properties. Appl Microbiol Biotechnol 2014;98:8927–8936 [CrossRef]
    [Google Scholar]
  3. Kavitha M. Cold active lipases – an update. Front Life Sci 2016;9:226–238 [CrossRef]
    [Google Scholar]
  4. Patkar S, Vind J, Kelstrup E, Christensen MW, Svendsen A et al. Effect of mutations in Candida antarctica B lipase. Chem Phys Lipids 1998;93:95–101 [CrossRef]
    [Google Scholar]
  5. Kim HR, Hou CT, Lee KT, Kim BH, Kim IH. Enzymatic synthesis of structured lipids using a novel cold-active lipase from Pichia lynferdii NRRL Y-7723. Food Chem 2010;122:846–849 [CrossRef]
    [Google Scholar]
  6. Bradner JR, Bell PJL, Te'o VSJ, Nevalainen KMH. The application of PCR for the isolation of a lipase gene from the genomic DNA of an Antarctic microfungus. Curr Genet 2003;44:224–230 [CrossRef]
    [Google Scholar]
  7. Mohammed S, Te’o J, Nevalainen H. A gene encoding a new cold-active lipase from an Antarctic isolate of Penicillium expansum. Curr Genet 2013;59:129–137 [CrossRef]
    [Google Scholar]
  8. Gianese G, Bossa F, Pascarella S. Comparative structural analysis of psychrophilic and meso- and thermophilic enzymes. Proteins 2002;47:236–249 [CrossRef]
    [Google Scholar]
  9. Jan AH, Dubreucq E, Drone J, Subileau M. A glimpse into the specialization history of the lipases/acyltransferases family of CpLIP2. Biochim Biophys Acta Proteins Proteom 1865;2017:1105–1113
    [Google Scholar]
  10. Vakhlu J, Kour A. Yeast lipases: enzyme purification, biochemical properties and gene cloning. Electron J Biotechnol 2006;9:69–85 [CrossRef]
    [Google Scholar]
  11. Buerth C, Kovacic F, Stock J, Terfrüchte M, Wilhelm S et al. Uml2 is a novel CalB-type lipase of Ustilago maydis with phospholipase A activity. Appl Microbiol Biotechnol 2014;98:4963–4973 [CrossRef]
    [Google Scholar]
  12. Imanishi Y, Yamazaki A, Nakase T. A new Barnettozyma species forming hat-shaped ascospores isolated from soil in Japan. J Gen Appl Microbiol 2010;56:447–453 [CrossRef]
    [Google Scholar]
  13. Kouker G, Jaeger KE. Specific and sensitive plate assay for bacterial lipases. Appl Environ Microbiol 1987;53:211–213
    [Google Scholar]
  14. Zhu H, Qu F, Zhu LH. Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride. Nucleic Acids Res 1993;21:5279–5280 [CrossRef]
    [Google Scholar]
  15. Kurtzman C, Robnett C. Phylogenetic relationships among yeasts of the 'Saccharomyces complex' determined from multigene sequence analyses. FEMS Yeast Res 2003;3:417–432 [CrossRef]
    [Google Scholar]
  16. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994;22:4673–4680 [CrossRef]
    [Google Scholar]
  17. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef]
    [Google Scholar]
  18. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef]
    [Google Scholar]
  19. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984;25:125–128 [CrossRef]
    [Google Scholar]
  20. Kurtzman CP, Fell JW, Boekhout T, Robert V.Methods for the isolation, phenotypic characterization and maintenance of yeasts In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011; pp87–110
    [Google Scholar]
  21. Imanishi Y, Ueda-Nishimura K, Mikata K. Two new species of Kazachstania that form ascospores connected by a belt-like intersporal body: Kazachstania zonata and Kazachstania gamospora. FEMS Yeast Res 2007;7:330–338 [CrossRef]
    [Google Scholar]
  22. Lachance MA. In defense of yeast sexual life cycles: the forma asexualis – An informal proposal. Yeast Newlett 2012;61:24–25
    [Google Scholar]
  23. Maldonado RR, Lopes DB, Aguiar-Oliveira E, Kamimura ES, Macedob GA. A review on Geotrichum lipases: production, purification, immobilization and applications. Chem Biochem Eng Q 2016;30:439–454 [CrossRef]
    [Google Scholar]
  24. Martinelle M, Holmquist M, Hult K. On the interfacial activation of Candida antarctica lipase A and B as compared with Humicola lanuginosa lipase. Biochim Biophys Acta 1995;1258:272–276 [CrossRef]
    [Google Scholar]
  25. Benjamin S, Pandey A. Candida rugosa lipases: molecular biology and versatility in biotechnology. Yeast 1998;14:1069–1087 [CrossRef]
    [Google Scholar]
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