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Volume 70, Issue 2

sp. nov., isolated from farmland soil Free

Abstract

A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and rod-shaped bacterium, designated as strain G-1, was isolated from farmland soil sampled in in Fuyang, Anhui Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain G-1 was closely related to 2-36 (97.7 % similarity). Strain G-1 contained iso-C, Cω6, iso-C and iso-C as the predominant fatty acids. The polar lipids of strain G-1 were diphosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid, an unidentified lipid and two unidentified glycolipids. The predominant respiratory quinone of strain G-1 was MK-9(H). The cell wall contained -diaminopimelic acid as the diagnostic diamino acid. The G+C content of the genomic DNA based on genome calculations was 64.2 mol%. Average nucleotide identity and the digital DNA–DNA hybridization values for the draft genomes between strain G-1 and strain 2-36 were 75.7 and 20.2 %, respectively. On the basis of phenotypic and phylogenetic data, strain G-1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is G-1 (=CCTCC AB2019021=KCTC 49258).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Cumulibacter soli sp. nov. , farmland soil and polyphasic taxonomy
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31670112)
    • Principle Award Recipient: Qing Hong
  • National Key R & D Program of China (Award 2017YFD0800702)
    • Principle Award Recipient: Qing Hong
  • Opening Fund of Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base (Award 028074911709)
    • Principle Award Recipient: Qing Hong
© 2020 The Authors
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2019-12-04
2024-04-20
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Cumulibacter soli sp. nov., isolated from farmland soil
Int J Syst Evol Microbiol 70, 1152 (2020); https://doi.org/10.1099/ijsem.0.003893
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