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Volume 70, Issue 2

sp. nov., a moderate halophile isolated from non-saline farm soil Free

Abstract

A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China. Growth occurred with 0.5–20 % (w/v) NaCl (optimum 4–7 %) at pH 5.5–11.0 (optimum pH 8.0) and at 20–50 °C (optimum 30–35 °C). Contained cell-wall peptidoglycan based on -diaminopimelic acid and possessed menaquinone-7 (MK-7) as the major respiratory isoprenoid quinone. The major cellular fatty acids were anteiso-C, anteiso-C and iso-C. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, five unidentified phospholipids and an unidentified glycolipid. The DNA G+C content was 44.1 mol%. Phylogeny based on 16S rRNA gene sequences indicated that strain JSM 102062 belonged to the genus , sharing high 16S rRNA gene sequence similarities to EN8d (99.4 %) and NHBX5 (98.3 %). The whole genomic analysis showed that strain JSM 102062 constituted a different taxon separated from the recognized species. Combined data from phenotypic and genotypic studies demonstrated that strain JSM 102062 represents a noval species of the genus , for which the name sp. nov. is proposed; the type strain is JSM 102062 (=CCTCC AB 2014166 = CGMCC 1.12957=DSM 28949=KCTC 33541).

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Keyword(s): halophilic , non-saline farm soil and Sediminibacillus terrae sp.nov.
© 2020 The Authors
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2019-11-21
2024-04-19
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Sediminibacillus terrae sp. nov., a moderate halophile isolated from non-saline farm soil
Int J Syst Evol Microbiol 70, 1139 (2020); https://doi.org/10.1099/ijsem.0.003892
/content/journal/ijsem/10.1099/ijsem.0.003892
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