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Volume 70, Issue 2

sp. nov., isolated from phycosphere microbiota of the toxin-producing dinoflagellate Free

Abstract

A Gram-negative, aerobic, non-motile, non-spore-forming and rod-shaped bacterium, named strain LZ-16-2, was isolated from the phycosphere microbiota of the paralytic shellfish poisoning toxin-producing marine dinoflagellate LZT09. Strain LZ-16-2 grew optimally at 28 °C at pH 6.5 and with 3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain LZ-16-2 fell within the genus and was most closely related to DSM 19537, with which the new isolate exhibited 98.5 % 16S rRNA gene sequence similarity. The major respiratory quinone was Q-8. The predominant cellular fatty acids were C ω8, summed feature 3 (C ω7 and/or C ω6), summed feature 8 (C ω7 and/or C ω6), C ω6, C 3-OH and C. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The average nucleotide identity and DNA–DNA genome hybridization relatedness values between strain LZ-16-2 and its closest relative, DSM 19537, were 92.8 and 55.1 %, respectively. The DNA G+C content was 61.3 mol%. Differential phenotypic properties and phylogenetic distinctiveness distinguished strain LZ-16-2 from all other members of the genus . On the basis of the polyphasic characterization, strain LZ-16-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LZ-16-2 (=KCTC 62344=CCTCC AB2017229).

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  • Accepted:
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Keyword(s): Alexandrium catenella , Haliea alexandrii sp. nov , paralytic shellfish poisoning toxins , phycosphere microbiota and toxic dinoflagellate
Funding
This study was supported by the:
  • Bureau of Science and Technology of Zhoushan (CN) (Award 2017C32083)
    • Principle Award Recipient: Qiao Yang
  • Talent Introduction Foundation of Zhejiang Ocean University
    • Principle Award Recipient: Qiao Yang
  • National Natural Science Foundation of China, http://dx.doi.org/10.13039/501100001809 (Award 41876114)
  • Natural Science Foundation of Zhejiang Province, http://dx.doi.org/10.13039/501100004731 (Award LY18D060007)
© 2020 The Authors
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2019-11-21
2024-04-20
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Haliea alexandrii sp. nov., isolated from phycosphere microbiota of the toxin-producing dinoflagellate Alexandrium catenella
Int J Syst Evol Microbiol 70, 1133 (2020); https://doi.org/10.1099/ijsem.0.003890
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