1887

Abstract

A moderately thermophilic and heterotrophic bacterial strain, LA, was isolated from microbial mats sampled at a hot spring in Nakabusa, Nagano, Japan. The cells of strain LA were aerobic, Gram-stain-negative, non-sporulating, non-motile and rod-shaped (2.0–4.1 µm long). The formation of dense cell aggregates in liquid medium was a unique characteristic of the strain. Strain LA grew optimally at 50°C and at pH 7.5. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the nearest neighbour of strain LA was JCM 30197 with 94.1 % sequence similarity. The draft genome sequence of strain LA (2 671 880 bp) showed 18.0 % digital DNA-DNA hybridization, 70.9 % average nucleotide identity and 72.1 % average amino acid identity (AAI) values in comparison with the genome sequence of JCM 30197 (2 606 763 bp); the 16S rRNA gene sequence similarity and AAI values are lower than the cutoffs used for assignment to a separate genus. On the basis of phenotypic features, major cellular fatty acid composition, genome sequencing and phylogenetic position, a novel genus and species are proposed for strain LA, to be named egans (= JCM 31826=DSM 106522).

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2019-11-28
2019-12-09
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