@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003885, author = "Kaewwichian, Rungluk and Khunnamwong, Pannida and Am-In, Somjit and Jindamorakot, Sasitorn and Limtong, Savitree", title = "Torulaspora nypae sp. nov., a novel yeast species isolated from nipa (Nypa fruticans Wurmb.) inflorescence sap in southern Thailand", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "2", pages = "1112-1116", doi = "https://doi.org/10.1099/ijsem.0.003885", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003885", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Torulaspora nypae sp. nov.", keywords = "new yeast species", keywords = "Thailand", keywords = "nipa inflorescence sap", abstract = "Two strains (YSP-384 and YSP-399), representing a novel Torulaspora species, were isolated from two nipa inflorescence sap samples collected in Trang province in the southern part of Thailand. The two strains had identical sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions. The two strains were closest to Torulaspora maleeae CBS 10694T, but with 1.1 % nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 5.2 % nucleotide substitutions in the ITS regions. Phylogenetic analysis based on the concatenated sequences of the ITS regions and the D1/D2 domains of the LSU rRNA gene supported that the two strains represented a distinct species in the genus Torulaspora. Some phenotypic characteristics of the two strains differed from T. maleeae including the two strains have ability to assimilate d-xylose, d-glucono-δ-lactone and melizitose, and inability to ferment maltose and raffinose, whereas T. maleeae has opposite results. Therefore, the two strains are described as representing a novel species, for which the name Torulaspora nypae sp. nov. was proposed.", }