@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003883, author = "Joshi, Amaraja and Thite, Sonia and Dhotre, Dhiraj and Moorthy, Manju and Joseph, Neetha and Ramana, V. Venkata and Shouche, Yogesh", title = "Nitrincola tapanii sp. nov., a novel alkaliphilic bacterium from An Indian Soda Lake", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "2", pages = "1106-1111", doi = "https://doi.org/10.1099/ijsem.0.003883", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003883", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Alkaliphilic bacteria", keywords = "Nitrincola", keywords = "Lonar Lake", abstract = "A novel Gram-stain-negative bacterial strain designated as MEB193T was isolated from a sediment sample collected from Lonar Lake, India. The cells were motile, non-spore-forming and rod-shaped. The strain was oxidase- and catalase-positive. It grew optimally at pH 9.0 and at 1 % (w/v) NaCl concentration at 30 °C. Based on 16S rRNA gene sequence similarity, MEB193T belongs to genus Nitrincola , with Nitrincola alkalilacustris ZV-19T (95.89 %) and Nitrincola lacisaponensis 4CAT (95.87 %) as its closest neighbours. The major fatty acid was summed feature 8 comprising C18:1ω7c/C18:1ω6c (52 %) followed by C16 : 0 (25 %). Phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG) were present as the major polar lipids. The draft genome obtained in this study was 2 793 747 bp and the G+C content was 50.79 mol%. Average nucleotide identity (71.76 %) and DNA–DNA hybridization (<20 %) values between strain MEB193T and Nitrincola lacisaponensis 4CAT confirmed the novelty of this new species. Based on phenotypic including chemotaxonomic and genotypic characterization data, strain MEB193T represents a new species of the genus Nitrincola for which the name Nitrincola tapanii sp. nov. is proposed. The type strain is MEB193T (=MCC 2863T=JCM 31570 T=KCTC 52390 T).", }