1887

Abstract

We performed a comparative genomic analysis to clarify the taxonomic relationship between the two species, and . Whole genome sequences of types strains of the two species became available recently. Average nucleotide identity (ANI) and DNA–DNA hybridization (isDDH) between the two type strains were determined. Type strains of the two species had a 97.25 % ANI and an 80.4 % isDDH value, which are above the well-recognized cutoffs (≥95–96 % ANI and ≥70 % isDDH) for bacterial species delineation. The two strains have similar overall phenotypic characteristics and are clustered together with high bootstrap values in the multi-locus sequence analysis on , , and housekeeping genes. It therefore becomes evident that the two species actually belong to the same species. has priority over , therefore we proposed that Duan . 2016 is a later heterotypic synonym of [ ]

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003871
2019-11-22
2019-12-09
Loading full text...

Full text loading...

References

  1. Mezzatesta ML, Gona F, Stefani S. Enterobacter cloacae complex: clinical impact and emerging antibiotic resistance. Future Microbiol 2012;7: 887– 902 [CrossRef]
    [Google Scholar]
  2. Brady C, Cleenwerck I, Venter S, Coutinho T, De Vos P. Taxonomic evaluation of the genus Enterobacter based on multilocus sequence analysis (MLSA): proposal to reclassify E. nimipressuralis and E. amnigenus into Lelliottia gen. nov. as Lelliottia nimipressuralis comb. nov. and Lelliottia amnigena comb. nov., respectively, E. gergoviae and E. pyrinus into Pluralibacter gen. nov. as Pluralibacter gergoviae comb. nov. and Pluralibacter pyrinus comb. nov., respectively, E. cowanii, E. radicincitans, E. oryzae and E. arachidis into Kosakonia gen. nov. as Kosakonia cowanii comb. nov., Kosakonia radicincitans comb. nov., Kosakonia oryzae comb. nov. and Kosakonia arachidis comb. nov., respectively, and E. turicensis, E. helveticus and E. pulveris into Cronobacter as Cronobacter zurichensis nom. nov., Cronobacter helveticus comb. nov. and Cronobacter pulveris comb. nov., respectively, and emended description of the genera Enterobacter and Cronobacter. Syst Appl Microbiol 2013;36: 309– 319 [CrossRef]
    [Google Scholar]
  3. Wu W, Wei L, Feng Y, Kang M, Zong Z. Enterobacter huaxiensis sp. nov. and Enterobacter chuandaensis sp. nov., recovered from human blood. Int J Syst Evol Microbiol 2019;69: 708– 714 [CrossRef]
    [Google Scholar]
  4. Duan YQ, Zhou XK, Di-Yan L, Li QQ, Dang LZ et al. Enterobacter tabaci sp. nov., a novel member of the genus Enterobacter isolated from a tobacco stem. Antonie van Leeuwenhoek 2015;108: 1161– 1169 [CrossRef]
    [Google Scholar]
  5. Rosselló-Móra R, Urdiain M, López-López A. DNA–DNA hybridization. Taxonomy of Prokaryotes 2011; 325– 347
    [Google Scholar]
  6. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009;106: 19126– 19131 [CrossRef]
    [Google Scholar]
  7. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018;68: 461– 466 [CrossRef]
    [Google Scholar]
  8. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015;25: 1043– 1055 [CrossRef]
    [Google Scholar]
  9. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014;64: 346– 351 [CrossRef]
    [Google Scholar]
  10. Wu W, Wei L, Feng Y, Kang M, Zong Z. Enterobacter huaxiensis sp. nov. and Enterobacter chuandaensis sp. nov., recovered from human blood. Int J Syst Evol Microbiol 2019;69: 708– 714 [CrossRef]
    [Google Scholar]
  11. Richter M, Rosselló-Móra R, Oliver Glöckner F, Peplies J. JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics 2016;32: 929– 931 [CrossRef]
    [Google Scholar]
  12. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017;110: 1281– 1286 [CrossRef]
    [Google Scholar]
  13. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013;14: 60 [CrossRef]
    [Google Scholar]
  14. Chun J, Rainey FA. Integrating genomics into the taxonomy and systematics of the bacteria and archaea. Int J Syst Evol Microbiol 2014;64: 316– 324 [CrossRef]
    [Google Scholar]
  15. Zhu B, Lou MM, Xie GL, Wang GF, Zhou Q et al. Enterobacter mori sp. nov., associated with bacterial wilt on Morus alba L. Int J Syst Evol Microbiol 2011;61: 2769– 2774 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003871
Loading
/content/journal/ijsem/10.1099/ijsem.0.003871
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error