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Volume 70, Issue 2

sp. nov., isolated from a culture of Free

Abstract

A Gram-stain-negative, yellow-pigmented, strictly aerobic bacterium, designated strain MaA-Y11, was isolated from a culture of in the Republic of Korea. Cells were non-motile rods showing catalase- and oxidase-positive reactions. Growth of strain MaA-Y11 was observed at 25–37 °C (optimum, 30 °C) and pH 6.0–9.0 (optimum, 7.0) and in the presence of 0–1.0 % (w/v) NaCl (optimum, 0 %). Strain MaA-Y11 did not produce flexirubin-type pigments. Strain MaA-Y11 contained iso-C, iso-C 3-OH and summed feature 3 (Cω7 and/or Cω6) as major cellular fatty acids and menaquinone-6 as the sole isoprenoid quinone. Phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids were detected as the major polar lipids. The G+C content of the genomic DNA was 37.0 mol%. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain MaA-Y11 formed a phyletic lineage with IP-10 within the genus . Strain MaA-Y11 was most closely related to IP-10, with a 98.85 % 16S rRNA gene sequence similarity, and shared less than 93.87 % sequence similarities with other type strains. Average nucleotide identity and DNA–DNA hybridization values between strain MaA-Y11 and the type strain of were 87.0 and 32.3 %, respectively. Here, we conclude that strain MaA-Y11 represents a new species of the genus , for which the name sp. nov. is proposed. The type strain is MaA-Y11 (=KACC 21225=JCM 33501).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Bacteroidetes , cyanobacterium , Flavobacterium microcysteis , Microcystis and new taxa
Funding
This study was supported by the:
  • Chung-Ang University (Award .)
    • Principle Award Recipient: Ye Lin Seo
  • National Institute of Biological Resources (Award NIBR No. 2019-02-001)
    • Principle Award Recipient: Che Ok Jeon
© 2020 The Authors
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2019-11-15
2024-04-25
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References

  1. Bernardet JF, Segers P, Vancanneyt M, Berthe F, Kersters K et al. Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis STROHL and Tait 1978). Int J Syst Bacteriol 1996; 46:128–148 [View Article]
     [Google Scholar]
  2. Jit S, Dadhwal M, Prakash O, Lal R. Flavobacterium lindanitolerans sp. nov., isolated from hexachlorocyclohexane-contaminated soil. Int J Syst Evol Microbiol 2008; 58:1665–1669 [View Article]
     [Google Scholar]
  3. Crawford RL, Mohn WW. Microbiological removal of pentachlorophenol from soil using a Flavobacterium . Enzyme Microb Technol 1985; 7:617–620 [View Article]
     [Google Scholar]
  4. Weon HY, Song MH, Son JA, Kim BY, Kwon SW et al. Flavobacterium terrae sp. nov. and Flavobacterium Cucumis sp. nov., isolated from greenhouse soil. Int J Syst Evol Microbiol 2007; 57:1594–1598 [View Article]
     [Google Scholar]
  5. Qu JH, Yuan HL, Li HF, Deng CP. Flavobacterium cauense sp. nov., isolated from sediment of a eutrophic lake. Int J Syst Evol Microbiol 2009; 59:2666–2669 [View Article]
     [Google Scholar]
  6. Kacagan M, Inan K, Belduz AO, Canakci S. Flavobacterium anatoliense sp. nov., isolated from fresh water, and emended description of Flavobacterium ceti . Int J Syst Evol Microbiol 2013; 63:2075–2081 [View Article]
     [Google Scholar]
  7. Ahn JH, Kim TW, Kim TS, Joung Y, Kim SB. Flavobacterium fluminis sp. nov. to accommodate an aerobic, halotolerant and gliding Flavobacterium isolated from freshwater. Int J Syst Evol Microbiol 2017; 67:3117–3121 [View Article]
     [Google Scholar]
  8. Lee Y, Jeon CO. Flavobacterium alvei sp. nov., isolated from a freshwater river. Int J Syst Evol Microbiol 2018; 68:1919–1924 [View Article]
     [Google Scholar]
  9. Li DD, Liu C, Zhang YQ, Wang XJ, Wang N et al. Flavobacterium arcticum sp. nov., isolated from Arctic seawater. Int J Syst Evol Microbiol 2017; 67:1070–1074 [View Article]
     [Google Scholar]
  10. Du J, Yi TH. Flavobacterium tyrosinilyticum sp. nov., isolated from the rhizosphere of wild strawberry. Int J Syst Evol Microbiol 2016; 66:2629–2634 [View Article]
     [Google Scholar]
  11. Ren Q, Yu M, Li Y, Zhang Y, Shi X et al. Flavobacterium ovatum sp. nov., a marine bacterium isolated from an Antarctic intertidal sandy beach. Int J Syst Evol Microbiol 2018; 68:795–800 [View Article]
     [Google Scholar]
  12. Sun JQ, Xu L, Liu M, Wang XY, Wu XL. Flavobacterium suaedae sp. nov., an endophyte isolated from the root of Suaeda corniculata . Int J Syst Evol Microbiol 2016; 66:1943–1949 [View Article]
     [Google Scholar]
  13. Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G. Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev 1971; 35:171–205
     [Google Scholar]
  14. Park YJ, Kim KH, Han DM, Lee DH, Jeon CO. Sphingobium terrigena sp. nov., isolated from gasoline-contaminated soil. Int J Syst Evol Microbiol 2019; 69:2459–2464 [View Article]
     [Google Scholar]
  15. Yoon SH, SM H, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically United database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617
     [Google Scholar]
  16. Nawrocki EP, Eddy SR. Query-dependent banding (QDB) for faster RNA similarity searches. PLoS Comput Biol 2007; 3:e56 [View Article]
     [Google Scholar]
  17. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article]
     [Google Scholar]
  18. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article]
     [Google Scholar]
  19. Lee I, Ouk Kim Y, Park SC, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article]
     [Google Scholar]
  20. Luo R, Liu B, Xie Y, Li Z, Huang W et al. SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. Gigascience 2012; 1:18 [View Article]
     [Google Scholar]
  21. Stackebrandt E, Frederiksen W, Garrity GM, Grimont PAD, Kämpfer P et al. Report of the AD hoc Committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 2002; 52:1043–1047 [View Article]
     [Google Scholar]
  22. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article]
     [Google Scholar]
  23. Gomori G. Preparation of buffers for use in enzyme studies. Methods Enzymol 1955; 1:138–146
     [Google Scholar]
  24. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P. editor Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp 607–654
     [Google Scholar]
  25. Fautz E, Reichenbach H. A simple test for flexirubin-type pigments. FEMS Microbiol Lett 1980; 8:87–91 [View Article]
     [Google Scholar]
  26. Lányi B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987; 19:1–67
     [Google Scholar]
  27. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  28. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  29. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977; 27:104–117 [View Article]
     [Google Scholar]
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Flavobacterium microcysteis sp. nov., isolated from a culture of Microcystis aeruginosa
Int J Syst Evol Microbiol 70, 1037 (2020); https://doi.org/10.1099/ijsem.0.003867
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