A Gram-stain-positive, yellow-pigmented, catalase-positive and oxidase-negative, strictly aerobic actinobacterium, designated strain YIM 131853T, was isolated from lichen collected from the South Bank of the Baltic Sea. The novel strain was non-spore-forming, short rod-shaped and motile with a single polar flagellum. The strain could grow at 4–37 °C (optimum, 28 °C), at pH 4.0–12.0 (pH 6.0) and at 0–3 % (w/v) NaCl (1 %). The DNA G+C content of strain YIM 131853T based on the draft genome sequence was 68.3 mol%. Predominant cellular fatty acids (>10 %) were identified as anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The polar lipid profile included diphosphatidylglycerol, dimannosyldiacylglycerol, three unknown glycolipids, two unknown phospholipids and one unknown lipid. Strain YIM 131853T had 2,4-diaminobutyric acid as the diagnostic cell-wall diamino acid, galactose and glucose as whole-cell sugars, and MK-10, MK-14, MK-13 and MK-12 as the major menaquinones. Although strain YIM 131853T exhibited a highest 16S rRNA gene sequence similarity (96.6 %) to Amnibacterium kyonggiense NBRC 109360T, phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain formed a tight lineage with Naasia aerilata NBRC 108725T (96.5 % 16S rRNA gene sequence similarity), which was the only species of genus Naasia. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain YIM 131853T should belong to the genus Naasia and represents a novel species of the genus Naasia, for which the name Naasia lichenicola sp. nov. is proposed. The type strain is YIM 131853T (=CGMCC 4.7565T=NBRC 113605T).
WeonHY,
KimSJ,
JangYH,
HamadaM,
TamuraT et al.Naasia aerilata gen. nov., sp. nov., a member of the family Microbacteriaceae isolated from air. Int J Syst Evol Microbiol2013; 63:2436–2441 [View Article]
HayakawaM,
NonomuraH.
Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment1987; 65:501–509 [View Article]
JiangY,
TangSK,
WieseJ,
XuLH,
ImhoffJF et al.Streptomyces hainanensis sp. nov., a novel member of the genus Streptomyces
. Int J Syst Evol Microbiol2007; 57:2694–2698 [View Article]
LiWJ,
XuP,
SchumannP,
ZhangYQ,
PukallR et al.Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol2007; 57:1424–1428 [View Article]
LiD,
LiuCM,
LuoR,
SadakaneK,
LamTW.
MEGAHIT: an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph. Bioinformatics2015; 31:1674–1676 [View Article]
DoetschRN.
Determinative methods of light microscopy. In
GerhardtP.
editor Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology; 1981 pp 21–33
XuP,
LiWJ,
TangSK,
ZhangYQ,
ChenGZ et al.Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol2005; 55:1149–1153 [View Article]
TindallBJ,
SikorskiJ,
SmibertRA,
KriegNR.
Phenotypic characterization and the principles of comparative systematics. In
ReddyCA,
BeveridgeTJ,
BreznakJA,
MarzlufGA,
SchmidtTM.
(editors) Methods for General and Molecular Microbiology Washington, DC: American Society for Microbiology; 2007
TangSK,
WangY,
ChenY,
LouK,
CaoLL et al.Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella
. Int J Syst Evol Microbiol2009; 59:2025–2032 [View Article]
MinnikinDE,
O'DonnellAG,
GoodfellowM,
AldersonG,
AthalyeM et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods1984; 2:233–241 [View Article]
KämpferP,
KroppenstedtRM.
Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol1996; 42:989–1005 [View Article]
NakagawaY,
YamasatoK.
Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol1993; 139:1155–1161 [View Article]
KimSJ,
LeeSS.
Amnibacterium kyonggiense gen. nov., sp. nov., a new member of the family Microbacteriaceae
. Int J Syst Evol Microbiol2011; 61:155–159 [View Article]