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Volume 70, Issue 2

sp. nov., isolated from gut of larva of Free

Abstract

A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea. Growth was observed at 15–50 °C (optimum, 28–37 °C), pH 6.0–8.0 (pH 7.0) and only without NaCl. 16S rRNA gene sequence comparisons indicated that strain FW100M-2 had the highest similarity to type strains of S3-4A (96.8 %) and DSM 1355 (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa. The phylogenetic tree showed that strain FW100M-2 fell into the genus , and formed a cluster with S3-4A independent from other species. Antesio-C, iso-C and anteiso-C were detected as the major fatty acids. The only isoprenoid quinone was MK-7. Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid were present. The -diaminopimelic acid was present in the cell-wall peptidoglycan. The genomic DNA G+C content was 51.5 mol%. Hence, strain FW100M-2 represents a novel species of the genus , for which the name sp. nov. is proposed, with FW100M-2 (=KACC 19327=NBRC 113071) as the type strain.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): gut , isoprenoid quinone , Paenibacillus protaetiae , polar lipid and Protaetia brevitarsis seulensis
© 2020 The Authors
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2019-11-08
2024-04-20
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Paenibacillus protaetiae sp. nov., isolated from gut of larva of Protaetia brevitarsis seulensis
Int J Syst Evol Microbiol 70, 989 (2020); https://doi.org/10.1099/ijsem.0.003860
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