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Volume 70, Issue 5

sp. nov., a new pathogen isolated from a patient with primary cutaneous nocardiosis Open Access

Abstract

A novel nocardioform strain, CICC 11023, was isolated from a tissue biopsy of neck lesions of a patient with primary cutaneous nocardiosis and characterized to establish its taxonomic position. The morphological, biochemical, physiological and chemotaxonomic properties of strain CICC 11023 were consistent with classification in the genus . Whole-cell hydrolysates were rich in meso-diaminopimelic acid, galactose, arabinose and fructose. Mycolic acids were present. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and two unidentified lipids, and the predominant menaquinone was cyclo MK-8 (H4, ω-cyclo). The main fatty acids (>5 %) were C 10-methyl (TBSA), C, summed feature 4 (C trans 9/C iso 2OH), C and C 10-methyl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the isolate is most closely related (>98 % similarity) to the type strains OFN 02.72, UI 122540 and YIM 30243, and phylogenetic analysis of gene sequences showed similarity (89.1–92.2 %) to NBRC 108936, IFM 0236 and IFM 0803. DNA–DNA hybridization results for strain CICC 11023 compared to type strains ranged from 20.4 to 35.4 %. The genome of strain CICC 11023 was 8.78 Mbp with a G+C content of 67.4 mol% overall. The average nucleotide identity (ANI) values between strain CICC 11023 and YIM 30243 were low (OrthoANIu=77.47 %), and the ANI values between strain CICC 11023 and NBRC 108936 were low (OrthoANIu=83.75 %). Consequently, strain CICC 11023 represents a novel species on the basis of this polyphasic study, for which the name sp. nov. is proposed. The type strain is CICC 11023 (=KCTC 39837).

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  • Accepted:
  • Published Online:
Keyword(s): cutaneous nocardiosis , Nocardia colli sp. nov. and pathogen
Funding
This study was supported by the:
  • Special Coordination Funds of Science and Technology Department of Yunnan province and Kunming Medical University (Award 2010CD174)
    • Principle Award Recipient: Xiaoyun Wang
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2024-04-18
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Nocardia colli sp. nov., a new pathogen isolated from a patient with primary cutaneous nocardiosis
Int J Syst Evol Microbiol 70, 2981 (2020); https://doi.org/10.1099/ijsem.0.003856
/content/journal/ijsem/10.1099/ijsem.0.003856
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