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Volume 70, Issue 3

sp. nov., isolated from mangrove sediment Free

Abstract

A Gram-variable, aerobic, motile and irregular rod-shaped bacterium, designated HB172195, was isolated from a mangrove sediment sample collected from Bamen Bay mangrove forest, China. Cells of the strain were oxidase-negative but positive for catalase and nitrate reduction. Strain HB172195 was found to grow at 15–50 °C (optimum, 25–40 °C), pH 5.0–9.0 (optimum, pH 7.0) and in 1.0–11.0 % (w/v) NaCl (optimum, 3–6 %). Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-7 and the cell-wall peptidoglycan was -diaminopimelic acid. The predominant cellular fatty acids were anteiso-C, anteiso-C and Cω alcohol. The major polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and an unidentified phospholipid. The genomic DNA G+C content was 40.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to SW-72 (96.3%), KMM 3737 (96.2 %) and JSM 076093 (95.5 %). Based on polyphasic taxonomic characterization, strain HB172195 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is HB172195 (=CGMCC 1.16730=JCM 33411).

  • Received:
  • Accepted:
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Keyword(s): 16S rRNA gene sequence based phylogeny , Bacillus caeni sp.nov. and mangrove sediment
Funding
This study was supported by the:
  • Central Public-interest Scientific Institution Basal Research Fund of CATAS from Chinese Government (Award 19CXTD-32)
    • Principle Award Recipient: Huiqin Huang
  • Central Public-interest Scientific Institution Basal Research Fund of CATAS from Chinese Government (Award 1630052016011)
    • Principle Award Recipient: Huiqin Huang
  • Financial Fund of the Ministry of Agriculture and Rural Affairs of China (Award NFZX2018)
    • Principle Award Recipient: Yonghua Hu
© 2020 The Authors
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2020-02-26
2024-04-19
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Bacillus caeni sp. nov., isolated from mangrove sediment
Int J Syst Evol Microbiol 70, 1503 (2020); https://doi.org/10.1099/ijsem.0.003853
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