1887

Abstract

Two yellow-pigmented, Gram-stain-negative and rod-shaped bacterial strains, designated as RY24 and ZYY160, were isolated from rice. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strains RY24 and ZYY160 belonged to the genus , and the 16S rRNA gene sequence similarity was 100 % The DNA homology between the two strains was 99.7 %. The 16S rRNA and gene sequences of the two strains showed highest similarity values to CGMCC 1.3392 and DSM 15758 (sharing 99.31 and 94.34 %, respectively). The major fatty acids of two strains were identified as summed feature 8 (Cω7 and/or Cω6), C and summed feature 3 (Cω7 and/or Cω6), and the major respiratory quinone was identified as ubiquinone Q-9, which are typical chemotaxonomic features of members of the genus . The genomic DNA G+C contents of strains RY24 and ZYY160 were determined to be 64.25 and 64.21 mol%, respectively. The DNA–DNA relatedness and average nucleotide identity values between the two strains and their closely related type strains were below 36 and 90 %, which supported that RY24 and ZYY160 represent a novel species in the genus . Phylogenetic and chemotaxonomic evidence, together with phenotypic characteristics, showed that the two isolates constitute a novel species of the genus . The type strain is RY24 (JCM 33201=ACCC 61555), for which the name sp. nov. is proposed.

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2019-11-21
2019-12-11
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