RT Journal Article SR Electronic(1) A1 Filippova, Svetlana N. A1 Surgucheva, Natalya A. A1 Detkova, Ekaterina N. A1 Rakitin, Andrey L. A1 Beletsky, Aleksey V. A1 Grouzdev, Denis S. A1 Kolganova, Tatyana V. A1 Mulyukin, Andrey L.YR 2020 T1 Serinibacter arcticus sp. nov., isolated from a thawing ancient ice wedge JF International Journal of Systematic and Evolutionary Microbiology, VO 70 IS 2 SP 929 OP 934 DO https://doi.org/10.1099/ijsem.0.003848 PB Microbiology Society, SN 1466-5034, AB A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia). Colonies of strain K3-2T were yellowish orange; cells had the fine structure typical of Gram-positive bacteria, were non-motile short rods and were non-spore-forming. Strain K3-2T was mesophilic (optimum growth at 28 °С), but capable of growing at 4 °С. The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine. The polar lipids were phosphatidylglycerol, lysophosphatidylserine, three unidentified phospholipids and glycolipids. The major fatty acids were anteiso-C15 : 0 and C16 : 0. The only menaquinone detected was MK-8(H4). 16S rRNA gene analysis indicated that strain K3-2T belongs to the genus Serinibacter . The closest taxonomically described relatives were Serinibacter salmoneus Kis4-28T and Serinibacter tropicus PS-14-7T, with 97.20 and 97.20 % 16 s rRNA gene sequence similarity, respectively. The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T was 78.9 %. DNA–DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T) were 41 and 47 %. Thus, strain K3-2T represents a novel species of the genus Serinibacter for which the name Serinibacter arcticus sp. nov. is proposed. The type strain is K3-2T (DSM 103859T=VKM Ас−2719T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003848