1887

Abstract

A novel actinobacterium, strain K3-2, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia). Colonies of strain K3-2 were yellowish orange; cells had the fine structure typical of Gram-positive bacteria, were non-motile short rods and were non-spore-forming. Strain K3-2 was mesophilic (optimum growth at 28 °С), but capable of growing at 4 °С. The cell-wall peptidoglycan of strain K3-2 contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine. The polar lipids were phosphatidylglycerol, lysophosphatidylserine, three unidentified phospholipids and glycolipids. The major fatty acids were anteiso-C and C. The only menaquinone detected was MK-8(H). 16S rRNA gene analysis indicated that strain K3-2 belongs to the genus . The closest taxonomically described relatives were Kis4-28 and PS-14-7, with 97.20 and 97.20 % 16 s rRNA gene sequence similarity, respectively. The average nucleotide identity value of the whole genome sequence between strain K3-2 and Kis4-28 was 78.9 %. DNA–DNA relatedness values between strain K3-2 and DSM 21801 (=Kis4-28) and VKPM Ac 2044 (=PS-14-7) were 41 and 47 %. Thus, strain K3-2 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is K3-2 (DSM 103859=VKM Ас−2719).

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2019-11-15
2019-12-11
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