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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 2

sp. nov. and sp. nov., isolated from the Southern Atlantic Ocean Free

Abstract

Two Gram-positive, aerobic, non-motile and non-spore-forming actinobacteria, strains GY074 and GY239, were isolated from deep-sea sediment of the Southern Atlantic Ocean. The results of phylogenetic analysis of 16S rRNA gene sequences placed both isolates within the genus , and showed a sequence similarity of 98.3 % between the two strains and similarites of 94.3–97.2 % with respect to species with validly published names. Based on whole-genome sequences, the values of DNA–DNA hybridization and the average nucleotide identity between strains GY074 and GY239 were 21.2 and 78.1 %, respectively, less than the proposed cut-off level for species delineation, i.e. 70 and 95 %. For both strains, the major cellular fatty acids were anteiso-C and anteiso-C, and the major menaquinones were MK-7, MK-8 and MK-9. The major polar lipid contents of the two strains were similar with phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid. The genomic DNA G+C contents of strains GY074 and GY239 were 61.1 and 64.2 mol%, respectively. On the basis of the phylogenetic analysis and physiological and chemotaxonomic data, the isolates represent two novel species of the genus , for which the names sp. nov. (type strain GY074=KCTC 39639=MCCC 1A11256) and sp. nov. (type strain GY239=KCTC 39640=MCCC 1A10688) are proposed.

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Keyword(s): marine sediment , Nesterenkonia salmonea sp. nov. , Nesterenkonia sphaerica sp. nov. and polyphasic taxonomy
Funding
This study was supported by the:
  • the Scientific Research Foundation of Third Institute of Oceanography, MNR (Award 2019011)
    • Principle Award Recipient: Gaiyun Zhang
  • China Ocean Mineral Resources R&D Association (COMRA) Program (Award DY135-B2-01)
    • Principle Award Recipient: Gaiyun Zhang
© 2020 The Authors
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2019-11-12
2025-03-17
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Nesterenkonia salmonea sp. nov. and Nesterenkonia sphaerica sp. nov., isolated from the Southern Atlantic Ocean
Int J Syst Evol Microbiol 70, 923 (2020); https://doi.org/10.1099/ijsem.0.003847
/content/journal/ijsem/10.1099/ijsem.0.003847
/content/journal/ijsem/10.1099/ijsem.0.003847
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