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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 2

sp. nov., isolated from a marine sand Free

Abstract

A Gram-stain-negative, aerobic, non-spore-forming, motile by gliding and rod-shaped bacterial strain, designated HSMS-1, was isolated from a marine sand collected from the Yellow Sea, Republic of Korea, and identified by a polyphasic taxonomic approach. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that HSMS-1 fell within the clade comprising the type strains of species of the genus . HSMS-1 exhibited 16S rRNA gene sequence similarity values of 99.0 and 98.7 % to the type strains of and and of 93.3–98.5 % to the type strains of the other species of the genus . The ANI and dDDH values between HSMS-1 and the type strains of , , , , and were 72.6–79.3 % and 17.4–22.2 %, respectively. Mean DNA–DNA relatedness value between HSMS-1 and the type strain of was 18 %. HSMS-1 contained MK-6 as the predominant menaquinone and iso-C, anteiso-C, iso-C 3-OH and iso-C as the major fatty acids. The major polar lipid of HSMS-1 was phosphatidylethanolamine. The DNA G+C content of HSMS-1 from genomic sequence data was 39.2 %. Distinguishing phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that HSMS-1 is separated from recognized species of the genus . On the basis of the data presented, strain HSMS-1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HSMS-1(=KACC 19899=NBRC 113648).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): genome , Gramella sabulilitoris , marine sand , novel species and polyphasic taxonomy
Funding
This study was supported by the:
  • Rural Development Administration (Award PJ013743)
    • Principle Award Recipient: Jung-Hoon Yoon
  • National Institute of Biological Resources (Award project on survey of indigenous species of Korea)
    • Principle Award Recipient: Jung-Hoon Yoon
© 2020 The Authors
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2019-11-12
2025-01-21
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Gramella sabulilitoris sp. nov., isolated from a marine sand
Int J Syst Evol Microbiol 70, 909 (2020); https://doi.org/10.1099/ijsem.0.003845
/content/journal/ijsem/10.1099/ijsem.0.003845
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