@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003841, author = "Li, Yong and Bian, Dan-ran and Chang, Ju-pu and Guo, Li-min and Yang, Xu-qi", title = "Sphingomonas populi sp. nov., isolated from bark of Populus × euramericana", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "2", pages = "897-901", doi = "https://doi.org/10.1099/ijsem.0.003841", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003841", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "new taxon", keywords = "populi", keywords = "endophytic bacteria", keywords = "Sphingomonas", abstract = "One Gram-stain-negative, aerobic, motile bacterial strain, 3-7T, was isolated from symptomatic canker bark tissue of Populus × euramericana. 16S rRNA gene sequence data revealed that the novel isolate shared highest similarity with Sphingomonas panacis DCY99T (98.8 %), and <96.5 % sequence similarity with all other species with validly published names. Growth occurred between 15 and 37 °C and at pH 6.0–9.0, and optimal growth occurred at 30 °C and pH 7.0–8.0. Nitrogen was produced from nitrates. The strain was positive for acetoin production and activity of catalase, oxidase, β-galactosidase, arginine dihydrolase and β-glucosidase. The major fatty acids were C16 : 0, C18 : 1ω7c and/or C18 : 1ω6c. The polar lipids of the novel isolate included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, sphingoglycolipid, glycolipid, two uncharacterized phospholipids and two uncharacterized lipids. The respiratory quinones detected in isolate 3-7T were Q-10 (96.9 %) and Q-9 (3.1 %). The DNA G+C content was 65.1 mol%. Based on phenotypic and genotypic characteristics, the isolate represents a novel species within the genus Sphingomonas , for which the name Sphingomonas populi is proposed. The type strain is 3-7T (=CFCC 11561T=LMG 30138T).", }