1887

Abstract

One Gram-stain-negative, aerobic, motile bacterial strain, 3-7, was isolated from symptomatic canker bark tissue of . 16S rRNA gene sequence data revealed that the novel isolate shared highest similarity with DCY99 (98.8 %), and <96.5 % sequence similarity with all other species with validly published names. Growth occurred between 15 and 37 °C and at pH 6.0–9.0, and optimal growth occurred at 30 °C and pH 7.0–8.0. Nitrogen was produced from nitrates. The strain was positive for acetoin production and activity of catalase, oxidase, -galactosidase, arginine dihydrolase and -glucosidase. The major fatty acids were C, Cω7 and/or Cω6. The polar lipids of the novel isolate included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, sphingoglycolipid, glycolipid, two uncharacterized phospholipids and two uncharacterized lipids. The respiratory quinones detected in isolate 3-7 were Q-10 (96.9 %) and Q-9 (3.1 %). The DNA G+C content was 65.1 mol%. Based on phenotypic and genotypic characteristics, the isolate represents a novel species within the genus , for which the name is proposed. The type strain is 3-7 (=CFCC 11561=LMG 30138).

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2019-11-28
2019-12-11
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