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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 2

Description of sp. nov., an emergent fish pathogen, and assessment of virulence using a tiger barb () infection model Free

Abstract

A recently described emergent disease of ornamental fish has been associated with an species positive for the surface protective antigen () C gene. Whole genome sequencing was performed on five isolates from diseased ornamental fish. In addition, these isolates were compared to and other -positive species isolated from terrestrial and marine mammals, birds and fish using multi-locus sequence analysis (MLSA). The genomes of fish pathogenic isolates were genetically distinct from , sharing 86.61–86.94 % average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values of 31.6–32.2 %, but 99.01–99.11 % ANI and 90.8–91.9 % dDDH values with the uncharacterized -positive sp. strain 2 isolated from swine. The findings indicate the -positive fish and swine isolates are conspecific and represent a previously unrecognized taxon. While phylogenies inferred from MLSA sequences confirm this conclusion, slight genetic differences between the fish isolates and swine strain 2 were indicated. Bath immersion challenge trials were conducted using tiger barbs () exposed by immersion to 10 c.f.u. ml of three fish pathogenic species, and three and two isolates as a model of infection. Thirty days post-challenge, cumulative mean percentage survival was 37 % for the , 100 % for the and 13 % for the isolates, revealing differences in virulence among the various genotypes in fish. Genetic findings and observed differences in virulence demonstrate the fish pathogenic isolates represent a novel species, for which the name sp. nov. is proposed. The type strain is 15TAL0474 (=NRRL B-65533=ATCC-TSD-175=DSM 110099).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): aquaculture , emergent and ornamental
Funding
This study was supported by the:
  • This work received no specific grant from any funding agency (Award Not applicable)
    • Principle Award Recipient: Not Applicable
© 2020 The Authors
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2019-11-04
2024-04-19
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Description of Erysipelothrix piscisicarius sp. nov., an emergent fish pathogen, and assessment of virulence using a tiger barb (Puntigrus tetrazona) infection model
Int J Syst Evol Microbiol 70, 857 (2020); https://doi.org/10.1099/ijsem.0.003838
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