1887

Abstract

Two novel strains of members of the genus , WCHEs120002 and WCHEs120003, were recovered from the sputum of two patients at a hospital in PR China in 2017. The strains were Gram-stain-negative, facultatively anaerobic, motile and non-spore-forming. The two strains were subjected to whole-genome sequencing. Phylogenetic analysis based on core genes of type strains of species of the family revealed that the two strains belonged to the genus r but were distinct from any previously known species of the genus. Both average nucleotide identity and DNA–DNA hybridization values between strains WCHEs120002 and WCHEs120003 and type strains of all known species of the genus were lower than the recommended thresholds of 95 and 70  %, respectively, for species delineation. The major fatty acids of the two strains were C, C cyclo and Cω7, which are similar to those of other species of the genus . Genomic DNA G+C contents of strains WCHEs120002 and WCHEs120003 were 56.09 and 55.91 mol%, respectively. WCHEs120002 ferments melibiose and sucrose but is negative for -sorbitol and methyl-α--mannopyranoside reactions, which distinguish it from all other species of the genus . WCHEs120003 can be differentiated from other species of the genus by its ability to ferment potassium gluconate and its negative reactions for -sorbitol and -fucose. Genotypic and phenotypic characteristics indicate that strains WCHEs120002 and WCHEs120003 represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed, respectively. The type strain of sp. nov. is WCHEs120002 (=GDMCC1.1569=NCTC 14273) and the type strain of sp. nov. is WCHEs120003 (=GDMCC1.1568=NCTC 14274).

Keyword(s): genome , sputum and Enterobacter
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/content/journal/ijsem/10.1099/ijsem.0.003837
2019-11-08
2019-11-21
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