1887

Abstract

A Gram-stain-negative, rod-shaped, non-motile, poly-β-hydroxybutyrate-accumulating and aerobic bacterial strain, designated CHR27, was isolated and characterized by using the polyphasic taxonomy approach. The results of phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of an up-to-date bacterial core gene set (92 protein clusters) indicated that strain CHR27 is affiliated with species in the genus . 16S rRNA gene sequence similarity results indicated that strain CHR27 was closely related to species of the genus (94.3–97.0 %), and had the highest sequence similarity to X23 (97.0 %). Strain CHR27 showed 19.4–22.1 % digital DNA–DNA hybridization values and 73.2–74.8 % average nucleotide identity values with the strains of other species. Optimal growth occurred at 25 °C, pH 7.5 and in the absence of NaCl. The major fatty acids of strain CHR27 were Cω7 and summed feature 3 (Cω7 and/or Cω6). The predominant hydroxy fatty acid was C 2-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, two unidentified sphingoglycolipids and an unidentified aminophospholipid. Strain CHR27 contained spermidine as the major polyamine and putrescine as a minor component. The only isoprenoid quinone was ubiquinone-10. The genomic DNA G+C content of strain CHR27was 61.8 mol%. On the basis of the phylogenetic inference and phenotypic data, strain CHR27 was considered a representative of a novel species within the genus . The name sp. nov. is proposed, with strain CHR27 (=BCRC 81121=LMG 30596=KCTC 62510) as the type strain.

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2019-11-01
2021-10-25
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