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Volume 70, Issue 2

sp. nov., isolated from the gut of the plastic-eating mealworm L. Free

Abstract

A bacterial strain, BIT-26, was isolated from the gut of plastic-eating mealworm L. The taxonomic position of this new isolate was investigated by using a polyphasic approach. Cells of the strain were Gram-stain-negative, facultatively anaerobic, motile rods with peritrichous flagella. The 16S rRNA gene sequence (1412 bp) of strain BIT-26 showed the highest similarity (97.4 %) to CFBP 5888, followed by NBRC 105722 (97.3 %), LMG 25383 (97.3 %), ATCC 51329 (97.2 %) and QC88-366 (97.2 %). The results of phylogenetic analyses, based on the 16S rRNA gene and concatenated sequences of four housekeeping genes (, , and ), placed strain BIT-26 within the genus of the family . This affiliation was also supported by the chemotaxonomic data. Strain BIT-26 had similar predominant fatty acids, including C, C, C, C cyclo and C cyclo 8, to species of the genus . DNA–DNA hybridization and average nucleotide identity calculations plus physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain BIT-26 from other species of the genus with validly published names. Therefore, strain BIT-26 is considered to represent a novel species, for which the name sp. nov is proposed. The type strain is BIT-26 (=CGMCC 1.17041=KCTC 72449).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): gut bacteria , mealworm , mixta , Plastic biodegradation and polystyrene
Funding
This study was supported by the:
  • Beijing Institute of Technology Research Fund Program for Young Scholars (Award 3160011181804)
    • Principle Award Recipient: Yu Yang
  • Young Elite Scientist Sponsorship Program of the China Association of Science and Technology (Award 2017QNRC001)
    • Principle Award Recipient: Yu Yang
  • National Natural Science Foundation of China (Award 31961133015)
    • Principle Award Recipient: Yu Yang
  • National Natural Science Foundation of China (Award 51603004)
    • Principle Award Recipient: Yu Yang
© 2020 The Authors
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2019-11-01
2024-04-19
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Mixta tenebrionis sp. nov., isolated from the gut of the plastic-eating mealworm Tenebrio molitor L.
Int J Syst Evol Microbiol 70, 790 (2020); https://doi.org/10.1099/ijsem.0.003826
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