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Volume 70, Issue 1

sp. nov., a novel endophytic actinobacterium isolated from bark of L. Free

Abstract

A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium, designated strain M2KJ-4, was isolated from a surface-sterilized bark of L. collected from Xinpu in Guizhou, PR China and characterized using a polyphasic approach to determine its taxonomic position. M2KJ-4 grew optimally with 1 % (w/v) NaCl at 25 °C and pH 8.0. Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested. Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4represented a member of the genus and shared the highest 16S rRNA gene sequence similarity with DSM 10552 (Z78209) (98.95 %). The DNA GC content of M2KJ-4 was 70.6 mol%. The average nucleotide identity value and estimated DDH value between M2KJ-4 and the type strain of were 86.1 % and 30.2 %, respectively. The cell-wall peptidoglycan contained -diaminopimelic acid and MK-9(H) was the predominant menaquinone. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and unidentified lipids. The major fatty acids were C, 10-methyl C, C 2-OH and Cω9. On the basis of the results from phylogenetic, phenotypic and chemotaxonomic analysis, strain M2KJ-4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M2KJ-4 (=KCTC 49174=CGMCC 1.13666).

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  • Accepted:
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Keyword(s): Aeromicrobium , endophytic actinobacterium , novel species and polyphasic approach
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 81603079)
    • Principle Award Recipient: Li Tuo
  • National Natural Science Foundation of China (Award 81960642)
    • Principle Award Recipient: Li Tuo
  • Youth Science and technology personnel growth project of Guizhou Provincial Education Department (Award QianJiaoHe KY Zi[2016]200)
    • Principle Award Recipient: Li Tuo
  • PhD Start-up Fund of Zunyi Medical University (Award F-800)
    • Principle Award Recipient: Yun Liu
  • Bidding Project of Zunyi Medical University (Award F-614)
    • Principle Award Recipient: Yun Liu
© 2020 The Authors
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2020-02-03
2024-04-19
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Aeromicrobium endophyticum sp. nov., a novel endophytic actinobacterium isolated from bark of Melia azedaeach L.
Int J Syst Evol Microbiol 70, 693 (2020); https://doi.org/10.1099/ijsem.0.003822
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