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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 1

sp. nov., isolated from forest soil Free

Abstract

A taxonomic study was performed on strain GCJ02, which was isolated from forest soil from Baishan City, PR China. The bacterium was Gram-stain-positive, catalase-positive and weakly oxidase-positive, rod-shaped and non-motile. Growth was observed at salinities of 0–6.0 % and at temperatures of 4–26 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that GCJ02 represented a member of the genus , with the highest sequence similarity to SK1 (99.5 %) and TMN-42 (99.5 %), followed by CGMCC 1.5382 (97.7 %), and other species of the genus (96.4–96.9 %). The ANI and the DNA–DNA hybridization estimate values between GCJ02 and all type strains of species of the genus were 72.5–84.5 % and 19.6–28.7 %, respectively. The principal fatty acids (>10 %) of GCJ02 were anteiso-C(53.0 %) and anteiso-C (18.8 %). The G+C content of the chromosomal DNA was 68.4 mol%. The respiratory quinone was determined to be MK-10. Phosphatidylglycerol, diphosphatidylglycerol, glycolipid, and one unidentified phospholipid and three unidentified polar lipid were present. The combined genotypic and phenotypic data indicated that strain GCJ02 represents a novel species within the genus , for which the name sp. nov. is proposed, with the type strain GCJ02 (=MCCC 1K03549=JCM 32391).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Actinobacteria , Cryobacterium and novel species
Funding
This study was supported by the:
  • The Foundation of Hubei Provincial Department of Education (Award B2018040)
    • Principle Award Recipient: Chunjie Gong
© 2020 The Authors
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2020-02-03
2025-01-16
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Cryobacterium soli sp. nov., isolated from forest soil
Int J Syst Evol Microbiol 70, 675 (2020); https://doi.org/10.1099/ijsem.0.003820
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