sp. nov. isolated from toxic dinoflagellate, Free

Abstract

A novel short-rod-shaped bacterial strain with poly-β-hydroxybutyric acid granules inside, designated as Z7-4, was isolated from a culture of a marine dinoflagellate with palytoxin-producing capacity, OS06, collected from the East China Sea. Cells of Z7-4 were Gram-stain-negative, non-motile, strictly aerobic, 0.9–1.2 µm wide and 2.0–3.9 µm long. Growth occurred in 1–4 % (w/v) NaCl, at 15–37 °C and at pH 5.0–10.0, with optimum growth in 3.5 % (w/v) NaCl, at 30 °C and at pH 7.0. Analysis of 16S rRNA gene sequence revealed that Z7-4 shared the highest 16S rRNA gene sequence similarities with JCM 30752 (98.8 %), followed by KCTC 42144 (98.6 %) and KCTC 32417 (96.9 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that Z7-4 clearly represented a member of the genus within the family . The respiratory quinone of Z7-4 was identified as Q-10. Polar lipids of Z7-4 were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, three unidentified aminophospholipids and one unidentified phospholipid. The major fatty acids were summed feature 8 (Cω7 and/or Cω6) and C. The DNA G+C content of Z7-4 was 63.0 mol%. DNA–DNA hybridization values of the isolate against the closely related type strains were far below the 70 % limit for species delineation. The average nucleotide identity and DNA–DNA genome hybridization relatedness between Z7-4 and the closely related members of the genus , KCTC 42144 and KCTC 32417, were 91.1 and 46.3 %, and 79.3 and 19.4 %, respectively. On the basis of the results of polyphasic analysis, Z7-4 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is Z7-4 (=KCTC 62459=CCTCC AB 2017231)

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41876114)
  • Natural Science Foundation of Zhejiang Province (Award LY18D060007)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003816
2019-12-13
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/70/2/759.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003816&mimeType=html&fmt=ahah

References

  1. Rajasabapathy R, Mohandass C, Yoon JH, Dastager SG, Liu Q et al. Nioella nitratireducens gen. nov., sp. nov., a novel member of the family Rhodobacteraceae isolated from Azorean Island. Antonie van Leeuwenhoek 2015; 107:589–595 [View Article]
    [Google Scholar]
  2. Liu Y, Du J, Lai Q, Dong C, Shao Z. Nioella sediminis sp. nov., isolated from surface sediment and emended description of the genus Nioella . Int J Syst Evol Microbiol 2017; 67:1271–1274 [View Article]
    [Google Scholar]
  3. Cha IT, Cho ES, Park JM, Yeh JY, Seo MJ et al. Nioella aestuarii sp. nov., of the family Rhodobacteraceae, isolated from tidal flat. Int J Syst Evol Microbiol 2017; 67:5205–5210 [View Article]
    [Google Scholar]
  4. CK L, Huang R, Chou HN. Toxins of Prorocentrum spp. (Dinophyta) isolated from Taiwan. PhD dissertation. Taipei: National Taiwan University; 2001
  5. Fidalgo C, Henriques I, Rocha J, Tacão M, Alves A. Culturable endophytic bacteria from the salt marsh plant Halimione portulacoides: phylogenetic diversity, functional characterization, and influence of metal(loid) contamination. Environ Sci Pollut Res 2016; 23:10200–10214 [View Article]
    [Google Scholar]
  6. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBio-Cloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article]
    [Google Scholar]
  7. Kimura M. The Neutral Theory of Molecular Evolution Cambridge, UK: Cambridge University Press; 1983
    [Google Scholar]
  8. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article]
    [Google Scholar]
  9. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [View Article]
    [Google Scholar]
  10. Luo R, Liu B, Xie Y, Li Z, Huang W et al. SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. Gigascience 2012; 1:18 [View Article]
    [Google Scholar]
  11. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article]
    [Google Scholar]
  12. Doetsch RN. Determinative methods of light microscopy. In Gerdhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA. (editors) Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology; 1981 pp 21–33
    [Google Scholar]
  13. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the international committee on systematics of prokaryotes. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070
    [Google Scholar]
  14. Yang Y, Lin Y, Li C, Lin D, Tang K et al. Ponticoccus lacteus sp. nov. of the family Rhodobacteraceae, isolated from surface seawater. Int J Syst Evol Microbiol 2015; 65:1247–1250 [View Article]
    [Google Scholar]
  15. Buczolits S, Kämpfer P, Denner EBM, Vybiral D, Busse H-J et al. Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov. Int J Syst Evol Microbiol 2002; 52:445–456 [View Article]
    [Google Scholar]
  16. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI; 1990
    [Google Scholar]
  17. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  18. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977; 27:104–117 [View Article]
    [Google Scholar]
  19. Kates M. Isolation, Analysis, and Identification of Lipids Amsterdam: Techniques of Lipidology, North Holland Publishing Company; 1986
    [Google Scholar]
  20. Maderankova D, Jugas R, Sedlar K, Vitek M, Skutkova H. Rapid bacterial species delineation based on parameters derived from genome numerical representations. Comput Struct Biotechnol J 2019; 17:118–126 [View Article]
    [Google Scholar]
  21. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.003816
Loading
/content/journal/ijsem/10.1099/ijsem.0.003816
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed