@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003816, author = "Yang, Qiao and Jiang, Zhiwei and Zhou, Xin and Zhang, Ruonan and Wu, Yiru and Lou, Linghao and Ma, Zetao and Wang, Dongfang and Ge, Yaming and Zhang, Xiaoling and Yu, Xinwei", title = "Nioella ostreopsis sp. nov., isolated from toxic dinoflagellate, Ostreopsis lenticularis", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "2", pages = "759-765", doi = "https://doi.org/10.1099/ijsem.0.003816", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003816", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Ostreopsis lenticularis", keywords = "Nioella ostreopsis sp. nov.", keywords = "algal-associated bacterium", keywords = "marine dinoflagellate", abstract = "A novel short-rod-shaped bacterial strain with poly-β-hydroxybutyric acid granules inside, designated as Z7-4T, was isolated from a culture of a marine dinoflagellate with palytoxin-producing capacity, Ostreopsis lenticularis OS06, collected from the East China Sea. Cells of Z7-4T were Gram-stain-negative, non-motile, strictly aerobic, 0.9–1.2 µm wide and 2.0–3.9 µm long. Growth occurred in 1–4 % (w/v) NaCl, at 15–37 °C and at pH 5.0–10.0, with optimum growth in 3.5 % (w/v) NaCl, at 30 °C and at pH 7.0. Analysis of 16S rRNA gene sequence revealed that Z7-4T shared the highest 16S rRNA gene sequence similarities with Nioella aestuarii JCM 30752T (98.8 %), followed by Nioella sediminis KCTC 42144T (98.6 %) and Nioella nitratireducens KCTC 32417T (96.9 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that Z7-4T clearly represented a member of the genus Nioella within the family Rhodobacteraceae . The respiratory quinone of Z7-4T was identified as Q-10. Polar lipids of Z7-4T were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, three unidentified aminophospholipids and one unidentified phospholipid. The major fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. The DNA G+C content of Z7-4T was 63.0 mol%. DNA–DNA hybridization values of the isolate against the closely related type strains were far below the 70 % limit for species delineation. The average nucleotide identity and in silico DNA–DNA genome hybridization relatedness between Z7-4T and the closely related members of the genus Nioella , N. sediminis KCTC 42144T and N. nitratireducens KCTC 32417T, were 91.1 and 46.3 %, and 79.3 and 19.4 %, respectively. On the basis of the results of polyphasic analysis, Z7-4T is proposed to represent a novel species of the genus Nioella , for which the name Nioella ostreopsis sp. nov. is proposed. The type strain of Nioella ostreopsis is Z7-4T (=KCTC 62459T=CCTCC AB 2017231T)", }