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Abstract

A novel short-rod-shaped bacterial strain with poly-β-hydroxybutyric acid granules inside, designated as Z7-4, was isolated from a culture of a marine dinoflagellate with palytoxin-producing capacity, OS06, collected from the East China Sea. Cells of Z7-4 were Gram-stain-negative, non-motile, strictly aerobic, 0.9–1.2 µm wide and 2.0–3.9 µm long. Growth occurred in 1–4 % (w/v) NaCl, at 15–37 °C and at pH 5.0–10.0, with optimum growth in 3.5 % (w/v) NaCl, at 30 °C and at pH 7.0. Analysis of 16S rRNA gene sequence revealed that Z7-4 shared the highest 16S rRNA gene sequence similarities with JCM 30752 (98.8 %), followed by KCTC 42144 (98.6 %) and KCTC 32417 (96.9 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that Z7-4 clearly represented a member of the genus within the family . The respiratory quinone of Z7-4 was identified as Q-10. Polar lipids of Z7-4 were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, three unidentified aminophospholipids and one unidentified phospholipid. The major fatty acids were summed feature 8 (Cω7 and/or Cω6) and C. The DNA G+C content of Z7-4 was 63.0 mol%. DNA–DNA hybridization values of the isolate against the closely related type strains were far below the 70 % limit for species delineation. The average nucleotide identity and DNA–DNA genome hybridization relatedness between Z7-4 and the closely related members of the genus , KCTC 42144 and KCTC 32417, were 91.1 and 46.3 %, and 79.3 and 19.4 %, respectively. On the basis of the results of polyphasic analysis, Z7-4 is proposed to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is Z7-4 (=KCTC 62459=CCTCC AB 2017231)

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/content/journal/ijsem/10.1099/ijsem.0.003816
2019-12-13
2020-01-24
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