Strains HY041T and HY039 were oxidase- and Gram-stain-negative, catalase-positive, rod-shaped, non-motile, and facultatively anaerobic bacteria. They were isolated from the feces of bats of the Hipposideros and Taphozous spp. collected from Chongqing City and Guangxi province (PR China), respectively. Phylogenetic analysis based on the 16S rRNA gene and 463 core genes indicated that HY041T and HY039 represent members of the genus Apibacter, forming a clade with Apibacter adventoris wkB301T (95.2 % 16S rRNA gene sequence similarity) and Apibacter mensalis R-53146T (94.0 %). In silico DNA–DNA hybridization (isDDH) and average nucleotide identity (ANI) values of our isolates with the most closely related species were lower than the 70 % and 95–96 % threshold, respectively, in contrast to values above these two thresholds (isDDH value: 89.1 %; ANI value: 98.5 %) between strains HY041T and HY039. The novel isolates could grow on nutrient and MacConkey agar. HY041T and HY039 could produce β-galactosidase and N-acetyl-β-glucosaminidase, and utilize d-adonitol, d-mannose, gentiobiose, glucose and salicin. The major fatty acids (>10.0 %) of HY041T were iso-C17 : 0 3OH, iso-C15 : 0, C16 : 0, summed feature 9 (C16 : 0 10-methyl and/or iso-C17 : 1ω9c) and C16 : 0 3OH. Polar lipids included phosphatidylethanolamine, glycolipid, two unidentified aminolipids and four unidentified lipids. Menaquinone 6 (MK-6) was the sole respiratory quinone. On the basis of all analyses so far, strains HY041T and HY039 represent a novel species of the genus Apibacter, for which the name Apibacter raozihei sp. nov. is proposed. The type strain is HY041T (=CGMCC 1.16567T=JCM 33423T) with a genomic DNA G+C content of 32.2 mol%.
KwongWK,
MoranNA.
Apibacter adventoris gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from honey bees. Int J Syst Evol Microbiol2016; 66:1323–1329 [View Article]
PraetJ,
AertsM,
BrandtED,
MeeusI,
SmaggheG et al.Apibacter mensalis sp. nov.: a rare member of the bumblebee gut microbiota. Int J Syst Evol Microbiol2016; 66:1645–1651 [View Article]
GeX-Y,
LiJ-L,
YangX-L,
ChmuraAA,
ZhuG et al. Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor. Nature2013; 503:535–538 [View Article]
YoonS-H,
HaS-M,
KwonS,
LimJ,
KimY et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol2017; 67:1613–1617 [View Article]
YarzaP,
RichterM,
PepliesJ,
EuzebyJ,
AmannR et al. The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol2008; 31:241–250 [View Article]
ZhuW,
YangJ,
LuS,
X-hL,
JinD et al.Fudania jinshanensis gen. nov., sp. nov., isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) in China. Int J Syst Evol Microbiol2019; ijsem003586:001466–005026
KolaczkowskiB,
ThorntonJW.
Performance of maximum parsimony and likelihood phylogenetics when evolution is heterogeneous. Nature2004; 431:980–984 [View Article]
KimuraM.
A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol1980; 16:111–120 [View Article]
ZwicklDJ,
HolderMT.
Model parameterization, prior distributions, and the general time-reversible model in Bayesian phylogenetics. Syst Biol2004; 53:877–888 [View Article]
GuindonS,
GascuelO.
A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol2003; 52:696–704 [View Article]
LuoR,
LiuB,
XieY,
LiZ,
HuangW et al. SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. Gigascience2012; 1:18 [View Article]
PriceMN,
DehalPS,
ArkinAP.
FastTree: computing large minimum evolution trees with profiles instead of a distance matrix. Mol Biol Evol2009; 26:1641–1650 [View Article]
MehlenA,
GoeldnerM,
RiedS,
StindlS,
LudwigW et al. Development of a fast DNA-DNA hybridization method based on melting profiles in microplates. Syst Appl Microbiol2004; 27:689–695 [View Article]
RichterM,
Rosselló-MóraR.
Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A2009; 106:19126–19131 [View Article]
CollinsMD,
JONESD.
Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Bacteriol1980; 48:459–470 [View Article]
KroppenstedtRM.
Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr1982; 5:2359–2367 [View Article]