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Volume 70, Issue 1

sp. nov., the first aerobic bacteriochlorophyll -containing fresh water bacterium in the genus of the class Free

Abstract

A strictly aerobic, bacteriochlorophyll -containing betaproteobacterium designated strain W35, was isolated from a biofilm sampled at Tama River in Japan. The non-motile and rod-shaped cells formed pink-beige pigmented colonies on agar plates containing organic compounds, and showed an absorption maximum at 871 nm in the near-infrared region, typical for the presence of bacteriochlorophyll . The new bacterial strain is Gram-negative, and oxidase- and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain W35 was closely related to species in the genus . The closest phylogenetic relatives of strain W35 were B8 (97.9 % sequence similarity), B4 (97.2 %) and ABP-4 (97.0 %). The major cellular fatty acids were C ω7 (50.4 %), C (22.7 %), summed feature 8 (C ω7/C ω6; 9.7 %), C ω6 (5.5 %), C (5.3 %) and C 3OH (2.7 %). The respiratory quinone was ubiquinone-8. Predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The G+C content of the genomic DNA was 70.4 mol% (genome data) and 71.4 mol% (HPLC). The genome size of strain W35 is 6.1 Mbp and average nucleotide identity analysis indicated genome similarities of strain W35 and related type strains to be 78–79 %. The results of polyphasic comparisons showed that strain W35 was clearly distinguishable from other members of the genus . Therefore, we propose a new species in the genus , namely, sp. nov. The type strain is W35 (=DSM 106757=NBRC 111963). The description of the genus is also emended.

  • Received:
  • Accepted:
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  • Published Online:
Keyword(s): AAPB , aerobic anoxygenic phototrophic bacteria , bacteriochlorophyll , Betaproteobacteria , epilithic biofilm and river
© 2020 The Authors
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2020-02-03
2024-04-25
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Aquabacterium pictum sp. nov., the first aerobic bacteriochlorophyll a-containing fresh water bacterium in the genus Aquabacterium of the class Betaproteobacteria
Int J Syst Evol Microbiol 70, 596 (2020); https://doi.org/10.1099/ijsem.0.003798
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