1887

Abstract

An obligately anaerobic, Gram-stain-negative, rod or coccobacilli organism was isolated from a faecal sample of a healthy Japanese woman. In the 16S rRNA gene sequence analysis, strain 5BBH33 showed the highest 16S rRNA gene sequence similarity to YIT 11850 (95.9 %), ADV 1053.03 (94.3 %), DSM 19965 (93.1 %), DSM 15470 (92.5 %) and ATCC 33048 (91.4 %). The gene sequence analysis also revealed strain 5BBH33 had relatively low gene sequence similarities (74.4–85.3 %) to other species. Strain 5BBH33 showed 21.8–23.9 % DNA–DNA hybridization values with other species. In addition, the average nucleotide identity values between strain 5BBH33 and other species ranged from 68.7–74.2 %, indicating that this strain should be considered as new species based on whole-genome relatedness. Strain 5BBH33 was asaccharolytic and largely unreactive for commercial kit. However, its growth was enhanced by adding 1 % (w/v) succinate to the medium; strain 5BBH33 was able to decarboxylate succinate to propionate. The strain 5BBH33 genome contained the enzymes involved in succinate utilization. These results improve our understanding of succinate-utilizing bacteria. On the basis of the collected data, strain 5BBH33 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain of is 5BBH33 (=JCM 33369=DSM 109768).

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2019-10-29
2019-11-20
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