1887

Abstract

Strain F21, a marine, aerobic, Gram-negative, rod-shaped bacterium, was isolated from seashore sand sampled in Pohang, Republic of Korea. Cells of strain F21 were non-motile, catalase-positive, oxidase-positive, non-spore-forming and formed pinkish-red colonies on marine agar. The strain grew optimally at 37°C, pH 7 and in the presence of 2–3 % NaCl (w/v). Analysis of the 16S rRNA gene sequence of strain F21 revealed that it belonged to the genus , family , with similarity values of 98.1 and 96.8 % to DSM 16067 and IMSNU 14014, respectively. When comparing the genome sequence of F21 with those of the type strains of six species of the genus , the values obtained were below the thresholds for analyses of average nucleotide identity (71.8–92.7 %) and DNA–DNA hybridization using the Genome-to-Genome Distance Calculator (14.7–75.2 %). The DNA G+C content of strain F21 was 42.0 mol%. The chemotaxonomic characteristics of F21 included MK-7 as the predominant isoprenoid quinone, iso-C, iso-C 3-OH and summed feature 3 (Cω6 and/or Cω7) as major cellular fatty acids, and phosphatidylcholine and phosphatidylethanolamine as major polar lipids. On the basis of phenotypic and chemotaxonomic properties, phylogenetic distinctiveness, and genomic data, we named strain F21 as sp. nov. and proposed that strain F21 (=KEMB 2250–007= KCTC 72106=JCM 33187) in the genus represents a novel species.

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2019-12-13
2020-01-27
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